IDENTIFICATION OF BRUCELLA DNA IN LYMPH TISSUE FROM DEER (CERVUS ELAPHUS) AND WILD BOARS (SUS SCROFA) BY THE USE OF BCSP31 PCR AND AMOS-PCR

At first, a genus-specific PCR assay (BCSP31) to identify all known Brucella, generating, an amplicon of 223 bp, was used, Then, positive samples were analysed with AMOS-PCR, which enables us to identify selected biovars of B. abortus, B. melitensis, B. ovis, and B. suis. The BCSP31 PCR analysis revealed that 27 (11.5%) of 235 samples originating from wild boars were positive and they all were negative in AMOS-PCR. Twenty-five were identified as B. sins biovar 2 through bacteriological examination. On the other hand, none of the 183 samples from deer was positive in PCR or bacteriological examination. The obtained results can provide a better knowledge of the potential reservoirs of infection and also should lead to great progress in molecular epidemiology.