CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities

被引：33

作者：

Roy, Bhaskar ^{[1
]}

Zhao, Jing ^{[1
,2
]}

Yang, Chao ^{[1
]}

Luo, Wen ^{[1
]}

Xiong, Teng ^{[1
]}

Li, Yong ^{[1
]}

Fang, Xiaodong ^{[1
]}

Gao, Guanjun ^{[3
]}

Singh, Chabungbam O. ^{[4
]}

Madsen, Lise ^{[1
,2
,5
]}

Zhou, Yong ^{[1
]}

Kristiansen, Karsten ^{[1
,2
]}

机构：

[1] BGI Shenzhen, BGI Genom, Shenzhen, Peoples R China

[2] Univ Copenhagen, Dept Biol, Lab Genom & Mol Biomed, Copenhagen, Denmark

[3] Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China

[4] Zhejiang Univ, Coll Anim Sci, Inst Sericulture & Apiculture, Hangzhou, Zhejiang, Peoples R China

[5] Inst Marine Res, Bergen, Norway

来源：

FRONTIERS IN GENETICS | 2018年 / 9卷

基金：

中国国家自然科学基金;

关键词：

CRISPR; Cascade; 9; genome editing; off-target effects; gene targeting; RNA guided system; RNA-GUIDED ENDONUCLEASE; ONE-STEP GENERATION; KNOCK-IN; CAENORHABDITIS-ELEGANS; GENE KNOCKOUT; DNA CLEAVAGE; C; ELEGANS; HOMOLOGOUS RECOMBINATION; CRISPR-CAS9; NUCLEASES; TARGETED MUTAGENESIS;

D O I：

10.3389/fgene.2018.00240

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.

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页数：12

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