Integrated Microcapillary for Sample-to-Answer Nucleic Acid Pretreatment, Amplification, and Detection

被引：81
|
作者：
Zhang, Lu [1 ,2 ]
Zhang, Yi [1 ,2 ]
Wang, Chunyan [3 ]
Feng, Qiang [1 ,2 ]
Fan, Fei [4 ]
Zhang, Guojun [4 ]
Kang, Xixiong [4 ]
Qin, Xuzhen [5 ,6 ]
Sun, Jiashu [1 ,2 ]
Li, Yinghui [3 ]
Jiang, Xingyu [1 ,2 ]
机构：
[1] Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China
[2] Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
[3] China Astronaut Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Beijing 100094, Peoples R China
[4] Capital Med Univ, Beijing Tiantan Hosp, Lab Diag Ctr, Beijing 100050, Peoples R China
[5] Peking Union Med Coll Hosp, CAMS, Dept Clin Lab, Beijing 100730, Peoples R China
[6] PUMC, Beijing 100730, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 20期
关键词：
MEDIATED ISOTHERMAL AMPLIFICATION; CARE DIAGNOSTICS; POINT; CHIP; IMMUNOASSAY; ARRAY; GENE; LAMP;
D O I：
10.1021/ac503072a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This work develops an integrated microcapillary-based loop-mediated isothermal amplification (icLAMP) containing preloaded reagents and DNA extraction card, allowing for sample-to-answer screening of single nucleotide polymorphisms (SNPs) typing of the CYP2C19 gene from untreated blood samples with minimal user operation. With all reagents and the DNA extraction card preloaded inside the capillary, this icLAMP system can achieve on-site pretreatment, extraction, amplification, and detection of nucleic acids within 150 min, without the requirement for advanced instruments. As icLAMP technology carries many advantages such as disposability, easy operation, low cost, and reduced cross contamination and biohazard risks, we expect this system to have a great impact on point-of-care (POC) nucleic acid detection.
引用
收藏
页码：10461 / 10466
页数：6
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