Transcriptome Analysis of Hypertrophic Heart Tissues from Murine Transverse Aortic Constriction and Human Aortic Stenosis Reveals Key Genes and Transcription Factors Involved in Cardiac Remodeling Induced by Mechanical Stress

被引：8

作者：

Yu, Peng ^{[1
]}

Zhang, Baoli ^{[2
]}

Liu, Ming ^{[3
]}

Yu, Ying ^{[3
]}

Zhao, Ji ^{[2
]}

Zhang, Chunyu ^{[2
]}

Li, Yana ^{[2
]}

Zhang, Lei ^{[2
]}

Yang, Xue ^{[2
]}

Jiang, Hong ^{[2
]}

Zou, Yunzeng ^{[2
]}

Ge, Junbo ^{[2
]}

机构：

[1] Fudan Univ, Zhongshan Hosp, Fudan Inst Metab Dis, Dept Endocrinol & Metab, Shanghai, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Zhongshan Hosp, Shanghai Clin Bioinformat Res Inst,Shanghai Inst, Shanghai, Peoples R China

[3] Fudan Univ, Shanghai Med Coll, Zhongshan Hosp, Dept Gen Practice, Shanghai, Peoples R China

来源：

DISEASE MARKERS | 2019年 / 2019卷

基金：

中国国家自然科学基金;

关键词：

EXPRESSION; DNA; NETWORKS; MICROARRAY; GENOMICS; DATABASE; DISEASE;

D O I：

10.1155/2019/5058313

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background. Mechanical stress-induced cardiac remodeling that results in heart failure is characterized by transcriptional reprogramming of gene expression. However, a systematic study of genomic changes involved in this process has not been performed to date. To investigate the genomic changes and underlying mechanism of cardiac remodeling, we collected and analyzed DNA microarray data for murine transverse aortic constriction (TAC) and human aortic stenosis (AS) from the Gene Expression Omnibus database and the European Bioinformatics Institute. Methods and Results. The differential expression genes (DEGs) across the datasets were merged. The Venn diagrams showed that the number of intersections for early and late cardiac remodeling was 74 and 16, respectively. Gene ontology and protein-protein interaction network analysis showed that metabolic changes, cell differentiation and growth, cell cycling, and collagen fibril organization accounted for a great portion of the DEGs in the TAC model, while in AS patients' immune system signaling and cytokine signaling displayed the most significant changes. The intersections between the TAC model and AS patients were few. Nevertheless, the DEGs of the two species shared some common regulatory transcription factors (TFs), including SP1, CEBPB, PPARG, and NFKB1, when the heart was challenged by applied mechanical stress. Conclusions. This study unravels the complex transcriptome profiles of the heart tissues and highlighting the candidate genes involved in cardiac remodeling induced by mechanical stress may usher in a new era of precision diagnostics and treatment in patients with cardiac remodeling.

引用

页数：10