Genes required for mitotic spindle assembly in Drosophila S2 cells

被引：426
|
作者：
Goshima, Gohta
Wollman, Roy
Goodwin, Sarah S.
Zhang, Nan
Scholey, Jonathan M.
Vale, Ronald D.
Stuurman, Nico
机构：
[1] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA
[2] Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA 94158 USA
[3] Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA
[4] Marine Biol Lab, Physiol Course, Woods Hole, MA 02543 USA
来源：
SCIENCE | 2007年 / 316卷 / 5823期
关键词：
D O I：
10.1126/science.1141314
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
引用
收藏
页码：417 / 421
页数：5
相关论文
共 50 条
  • [1] Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells
    Moutinho-Pereira, Sara
    Stuurman, Nico
    Afonso, Olga
    Hornsveld, Marten
    Aguiar, Paulo
    Goshima, Gohta
    Vale, Ronald D.
    Maiato, Helder
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (49) : 19808 - 19813
  • [2] Assessment of Mitotic Spindle Phenotypes in Drosophila S2 Cells
    Goshima, Gohta
    [J]. MICROTUBULES: IN VIVO, 2010, 97 : 259 - 275
  • [3] Determinants of mitotic spindle length in Drosophila S2 cells
    Goshima, G
    Wollman, R
    George, OL
    Kunwar, PS
    Scholey, JM
    Vale, RD
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2004, 15 : 397A - 397A
  • [4] Teashirt is required for mitotic progression in Drosophila larval neuroblasts and S2 cells
    Pimentel, SA
    Salazar, IM
    Fasano, L
    Kerridge, S
    Gomes, R
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 309A - 309A
  • [5] Drosophila S2 Cells as a Model System to Investigate Mitotic Spindle Dynamics, Architecture, and Function
    Moutinho-Pereira, Sara
    Matos, Irina
    Maiato, Helder
    [J]. MICROTUBULES: IN VIVO, 2010, 97 : 243 - 257
  • [6] Transient expression of MFSV genes in Drosophila S2 cells
    Cisneros, F. M.
    Tsai, C.
    Whitfield, A. E.
    Hogenhout, S. A.
    Redinbaugh, M. G.
    [J]. PHYTOPATHOLOGY, 2010, 100 (06) : S184 - S184
  • [7] Accurate measurement of poleward microtubule flux in the spindle of Drosophila S2 cells
    Munzarova, Alina
    Popova, Julia
    Razuvaeva, Alena
    Shloma, Victor
    Gatti, Maurizio
    Omelyanchuk, Leonid
    [J]. CELL BIOLOGY INTERNATIONAL, 2016, 40 (09) : 984 - 990
  • [8] Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells
    Guilgur, Leonardo G.
    Prudencio, Pedro
    Ferreira, Tania
    Pimenta-Marques, Ana Rita
    Martinho, Rui Goncalo
    [J]. DEVELOPMENT, 2012, 139 (03): : 503 - 513
  • [9] Mad2-independent spindle assembly checkpoint activation and controlled metaphase-anaphase transition in Drosophila S2 cells
    Orr, Bernardo
    Bousbaa, Hassan
    Sunkel, Claudio E.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (03) : 850 - 863
  • [10] Mob4 plays a role in spindle focusing in Drosophila S2 cells
    Trammell, Matthew A.
    Mahoney, Nicole M.
    Agard, David A.
    Vale, Ronald D.
    [J]. JOURNAL OF CELL SCIENCE, 2008, 121 (08) : 1284 - 1292
12345