Enhanced screening of key ultrasonication parameters: total phenol content and antioxidant activity assessment of Tartary buckwheat (Fagopyrum tataricum) water extract

The purpose of this study was to come up with a method to screen ultrasonication parameters that mainly predict ultrasound extraction process efficiency, using multiple regression analysis and response surface method (RSM). The relevance of this study is the fact that combining RSM with multiple regression modeling gives a conclusive report on the actual effects of individual treatment parameters. Although all predictors [time (A), frequency (B), power (C) and ratio (D)] showed significance in RSM models, multiple regression models further indicated that only D significantly influenced the variability observed in total phenol content and antioxidant activity of buckwheat extracts.