Human meibomian gland epithelial cell culture models: Current progress, challenges, and future directions

被引:8
|
作者
Phan, Minh Anh Thu [1 ]
Madigan, Michele C. [1 ]
Stapleton, Fiona [1 ]
Willcox, Mark [1 ]
Golebiowski, Blanka [1 ]
机构
[1] UNSW Sydney, Sch Optometry & Vis Sci, Fac Med & Hlth, Kensington, NSW 2033, Australia
来源
OCULAR SURFACE | 2022年 / 23卷
基金
澳大利亚研究理事会;
关键词
Human meibomian gland epithelial cell; Meibocyte; 3D culture; Meibomian gland stem cells; Organoids; HUMAN MEIBUM; GENE-EXPRESSION; INTERNATIONAL WORKSHOP; TRANSCRIPTION FACTOR; FATTY-ACIDS; MEIBOCYTE DIFFERENTIATION; EICOSAPENTAENOIC ACID; ANDROGEN DEFICIENCY; HOLOCRINE SECRETION; CHOLESTERYL ESTERS;
D O I
10.1016/j.jtos.2021.11.012
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The widely used immortalised human meibomian gland epithelia cell (iHMGEC) line has made possible extensive studies of the biology and pathophysiology of meibomian glands (MG). Tissue culture protocols for iHMGEC have been revised and modified to optimise the growth conditions for cell differentiation and lipid accumulation. iHMGEC proliferate in serum-free medium but require serum or other appropriate exogenous factors to differentiate. Several supplements can enhance differentiation and neutral lipid accumulation in iHMGEC grown in serum-containing medium. In serum-free medium, rosiglitazone, a peroxisome proliferator activator receptor-gamma (PPAR gamma) agonist, is reported to induce iHMGEC differentiation, neutral lipid accumulation and expression of key biomarkers of differentiation. iHMGEC cultured in serum-containing medium under hypoxia or with azithromycin increases DNAse 2 activity, a biomarker of terminal differentiation in sebocytes. The production of lipids with composition similar to meibum has not been observed in vitro and this remains a major challenge for iHMGEC culture. Innovative methodologies such as 3D ex vivo culture of MG and generation of MG organoids from stem cells are important for further developing a model that more closely mimics the in vivo biology of human MG and to facilitate the next generation of studies of MG disease and dry eye.
引用
收藏
页码:96 / 113
页数:18
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