Expression of the phospho-β-glycosidase ArbZ from Lactobacillus delbrueckii subsp lactis in Lactobacillus helveticus:: substrate induction and catabolite repression

被引:4
|
作者
Weber, BA [1 ]
Klein, JR [1 ]
Henrich, B [1 ]
机构
[1] Univ Kaiserslautern, Fachbereich Biol, Abt Mikrobiol, D-67653 Kaiserslautern, Germany
来源
MICROBIOLOGY-SGM | 2000年 / 146卷
关键词
Lactobacillus delbrueckii; ArbZ phospho-beta-glycosidase; substrate induction; catabolite repression;
D O I
10.1099/00221287-146-8-1941
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
ArbZ from Lactobacillus delbrueckii subsp. lactis was previously shown to enable utilization of the beta-glucoside arbutin by Escherichia coli. The arbZ gene was cloned and expressed in the industrially used beta-glucoside-negative strain Lactobacillus helveticus 3036(62). The transformants were able to ferment not only arbutin, but also cellobiose, salicin and methyl-beta-glucoside (M beta Glc). Cleavage of beta-glucosides by the transformants depended on the integrity of the cytoplasmic membrane, whereas in cell-free extracts only C-6-phosphorylated substrates were hydrolysed, This suggested that ArbZ is a phospho-beta-glycosidase. ArbZ activity in transformants of Lb. helveticus was subject to substrate induction mediated by the beta-glucosides arbutin, salicin and M beta Glc, whereas cellobiose or the beta-galactoside lactose had no inducing effect. Northern blot analysis proved that induction by M beta Glc was due to enhanced transcription of arbZ. Catabolite repression of arbZ induction was observed with glucose, mannose, fructose and galactose. The induction kinetics observed in the presence of these sugars indicated that at least two different mechanisms are operative in catabolite repression of arbZ in Lb. helveticus.
引用
收藏
页码:1941 / 1948
页数:8
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