New Diketopyrrolopyrrole-Based Ratiometric Fluorescent Probe for Intracellular Esterase Detection and Discrimination of Live and Dead Cells in Different Fluorescence Channels

被引：64
|
作者：
Wang, Jian [1 ,2 ]
Xu, Weibo [3 ,4 ]
Yang, Zhicheng [1 ,2 ]
Yan, Yongchao [1 ,2 ]
Xie, Xiaoxu [1 ,2 ]
Qu, Ning [3 ,4 ]
Wang, Yu [3 ,4 ]
Wang, Chengyun [1 ,2 ]
Hua, Jianli [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China
[2] East China Univ Sci & Technol, Inst Fine Chem, Coll Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Dept Oncol, Shanghai 200032, Peoples R China
[4] Fudan Univ, Dept Head & Neck Surg, Shanghai Canc Ctr, Shanghai 200032, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2018年 / 10卷 / 37期
基金：
中国国家自然科学基金;
关键词：
diketopyrrolopyrrole; fluorescent probe; ratiometric; esterase; live and dead cells; DIFFERENT CONCENTRATION RANGES; NEAR-INFRARED REGION; ESIPT CHARACTERISTICS; SELECTIVE DETECTION; CONJUGATED POLYMER; SCHIFF-BASE; NANOPARTICLES; GLUTATHIONE; METABOLISM; EMISSION;
D O I：
10.1021/acsami.8b11365
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
A new diketopyrrolopyrrole-based fluorescent probe (DPP-AM) was designed and synthesized for ratiometric detection of esterase and for imaging of live and dead cells in different modes. DPP-AM showed red fluorescence because of the intramolecular charge transfer (ICT) process from the DPP moiety to the pyridinium cation and gave remarkable ratio changes (about 70 folds), with the fluorescence changing from red to yellow, after treating with esterase because of the broken ICT process. Besides, the detection limit of DPP-AM toward esterase in vitro was 9.51 X 10(-5) U/mL. After pretreating with H2O2 and ultraviolet light radiation, the health status of TPC1 cells was successfully imaged. More importantly, DPP-AM showed yellow fluorescence in live cells and a red fluorescent signal in dead cells, indicating that assessing esterase activity as well as for discriminating live and dead cells.
引用
收藏
页码：31088 / 31095
页数：8
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