Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor

被引：13

作者：

Bogachek, M. V. ^{[1
]}

Zaitsev, B. N. ^{[1
]}

Sekatskii, S. K. ^{[2
]}

Protopopova, E. V. ^{[1
]}

Ternovoi, V. A. ^{[1
]}

Ivanova, A. V. ^{[1
]}

Kachko, A. V. ^{[1
]}

Ivanisenko, V. A. ^{[3
]}

Dietler, G. ^{[2
]}

Loktev, V. B. ^{[1
]}

机构：

[1] FSRI State Res Ctr Virol & Biotechnol Vector, Koltsov 630559, Novosibirsk Reg, Russia

[2] Ecole Polytech Fed Lausanne, BSP, IPSB, Lab Phys Mat Vivante, CH-1015 Lausanne, Switzerland

[3] Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk 630090, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 04期

基金：

俄罗斯基础研究基金会; 瑞士国家科学基金会;

关键词：

West Nile virus; glycoprotein E; atomic force spectroscopy; alpha V beta 3 integrin; BORNE ENCEPHALITIS-VIRUS; DOMAIN-III; NEUTRALIZING ANTIBODIES; MONOCLONAL-ANTIBODIES; ANTIGENIC STRUCTURE; AFM TIP; PROTEIN; BINDING; SPECTROSCOPY; STRAIN;

D O I：

10.1134/S0006297910040115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant polypeptide containing the 260-466 amino acid sequence of West Nile virus (WNV) strain LEIV-Vlg99-27889-human glycoprotein E (gpE, E260-466) was constructed. Immunochemical similarity between the E260-466 and gpE of WNV was proven by enzyme immunoassay (EIA), immunoblot, competitive EIA, hemagglutination inhibition, and neutralization tests using polyclonal and monoclonal antibodies against the viral gpE and recombinant E260-466. Polypeptide E260-466 induced formation of virus neutralizing and cross-reactive antibodies that were interactive with various epitopes of this recombinant protein. It is shown by evaluation of the interaction of E260-466 with one of the proposed cell receptors of WNV that average E260-466-alpha V beta 3 integrin-specific interaction force measured using atomic force spectroscopy was 80 and 140 pN for single and double interactions, correspondingly. Taken together with previously described interaction between laminin-binding protein (LBP) and WNV gpE domain II, it is proposed that WNV gpE can interact specifically with two cellular proteins (LBP and alpha V beta 3 integrin) during virus entry.

引用

页码：472 / 480

页数：9

共 7 条

[1] Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with αVβ3 integrin as putative cellular receptor
M. V. Bogachek
B. N. Zaitsev
S. K. Sekatskii
E. V. Protopopova
V. A. Ternovoi
A. V. Ivanova
A. V. Kachko
V. A. Ivanisenko
G. Dietler
V. B. Loktev
Biochemistry (Moscow), 2010, 75 : 472 - 480
[2] Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81
Flint, M
Maidens, C
Loomis-Price, LD
Shotton, C
Dubuisson, J
Monk, P
Higginbottom, A
Levy, S
McKeating, JA
JOURNAL OF VIROLOGY, 1999, 73 (08) : 6235 - 6244
[3] Interaction of West Nile virus with αvβ3 integrin mediates virus entry into cells
Chu, JJH
Ng, ML
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (52) : 54533 - 54541
[4] Interaction of West Nile virus with αvβ3 integrin mediates virus entry into cells
Chu, Justin Jang-Hann
Ng, Mah-Lee
J. Biol. Chem., 1600, 52 (54533-54541):
[5] Quantifying the specific binding between West Nile virus envelope domain III protein and the cellular receptor αVβ3 integrin
Lee, JWM
Chu, JJH
Ng, ML
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (03) : 1352 - 1360
[6] Immunochernical and single molecule force spectroscopy studies of specific interaction between the laminin binding protein and the West Nile virus surface glycoprotein E domain II
Bogachek, Maria V.
Protopopova, Elena V.
Loktev, Valery B.
Zaitsev, Boris N.
Favre, Melanie
Sekatskii, Sergey K.
Dietler, Giovanni
JOURNAL OF MOLECULAR RECOGNITION, 2008, 21 (01) : 55 - 62
[7] The Hepatitis C Virus Envelope Protein, HCV-E2, Inhibits Interferon-α-Stimulated STAT3 Tyrosine Phosphorylation in Hepatocytes via Its Interaction With the CD81 Receptor, in a Protein Kinase C β-Dependent Manner
Mullins, Sarah
Norris, Suzanne
Long, Aideen
GASTROENTEROLOGY, 2011, 140 (05) : S899 - S900

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