Production of a thermostable alkali-tolerant xylanase from Bacillus circulans AB 16 grown on wheat straw

被引：31
|
作者：
Dhillon, A [1 ]
Khanna, S [1 ]
机构：
[1] Tata Energy Res Inst, India Habitat Ctr, New Delhi 110003, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2000年 / 16卷 / 04期
关键词：
Bacillus circulans; pulping; thermostability; wheat straw; xylanase;
D O I：
10.1023/A:1008911026606
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Bacillus circulans AB 16 was able to produce 50 IU/ml of xylanase, with negligible cellulase activity when grown on untreated wheat straw. The pH optimum of the crude enzyme was 6-7 with a temperature optimum of 80 degrees C. The enzyme showed high pH and thermal stability retaining 100% activity at 60 degrees C, pH 8 and 9 after 2.5 h of incubation. The residual activity at 70 degrees C after 2.5 h was 62% and 45% at pH 8 and 9, respectively. At 75 degrees C only 22.2% activity remained at pH 8 after 1 h incubation. Since Kraft pulp is alkaline this enzyme could be used for prebleaching of pulp at temperatures up to 70 degrees C without pH adjustment.
引用
收藏
页码：325 / 327
页数：3
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