Routine laboratory test enabling the detection of dermatophytes and the identification of Trichophyton rubrum by means of in-house duplex real-time PCR

被引：2
|
作者：
Pablo, Thomas [1 ]
Mzabi, Alexandre [1 ]
Meo, Marie [1 ]
Decruyenaere, Frederic [1 ]
Perrin, Monique [1 ]
机构：
[1] Lab Natl Sante, Dept Microbiol, Bacteriol Mycol Antibiot Resistance & Hosp Hyg Se, Dudelange, Luxembourg
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2021年 / 185卷
关键词：
Dermatophytes; Trichophyton rubrum; Pre-treatment PCR; Duplex real-time PCR; CFX96 (R); CONVENTIONAL METHODS; RDNA; NAIL; BLOT; SKIN;
D O I：
10.1016/j.mimet.2021.106229
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dermatophytes are responsible, in majority, for fungal infections of skin, hair and nails, and Trichophyton rubrum is the most frequently isolated dermatophyte in humans. The time for dermatophyte growth in culture requires a total of two to four weeks. Molecular methods were developed to improve time to diagnosis and initiation of treatment. We present here an in-house duplex real-time PCR enabling detection of dermatophytes and simultaneous identification of T. rubrum from mycological samples and cultures. The objective of this work was to optimize the fungal DNA extraction method, the detection of dermatophytes and the identification of T. rubrum on a CFX96 (R) (Real-Time PCR Detection System). In addition, the method comparison showed that this new method is more sensitive than the culture and microscopic observations. To conclude, this routinely used method has been accredited ISO 15189 since January 2020 in our laboratory.
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页数：4
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