Eleusine indica Inhibits Early and Late Phases of Herpes Simplex Virus Type 1 Replication Cycle and Reduces Progeny Infectivity

被引:3
|
作者
Iberahim, Rashidah [1 ]
Nor, Norefrina Shafinaz Md [1 ]
Yaacob, Wan Ahmad [2 ]
Ibrahim, Nazlina [1 ]
机构
[1] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Biosci & Biotechnol, Ukm Bangi 43600, Selangor Darul, Malaysia
[2] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Chem Sci & Food Technol, Ukm Bangi 43600, Selangor Darul, Malaysia
来源
SAINS MALAYSIANA | 2018年 / 47卷 / 07期
关键词
Eleusine indica methanol extract; HHV1 replication cycle; progeny infectivity; IN-VITRO; ACIDS; HERPESVIRUSES; FLAVONOIDS; FUSION; GENES; CELLS; GB; GD;
D O I
10.17576/jsm-2018-4707-10
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The present study was aimed at determining the compounds available in Eleusine indica methanol extract and the effects on herpes simplex virus type 1 (HHV1) replication cycle and progeny infectivity. Twelve compounds mostly from the flavonoid and phenolic groups were identified by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analysis. The effect on replication phases of HHV1 was determined by time-of-addition, time-removal and virus yield reduction assays with expression of selected genes analysed by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The extract inhibited plaque formation the most during the first 2 h and at 24 h of infection. Plaque formation inhibition was also noted at all other time points but at lesser percentage. Treatment with E. indica reduced progeny infectivity when treated for 10 h and was dose-dependent. E. indica methanol extract inhibited immediate early, early and late phases of HHV1 replication cycle by modifying the expression of U(L)54, U(L)27 and U(L)30 genes during the infection. Inmmunostaining of infected cells confirmed that E. indica inhibited mainly Glycoproteins B but not Glycoprotein C and D. Thus, the methanol extract of E. indica has the ability to alter HHV1 replication cycle at almost all stages and reduce progeny infectivity.
引用
收藏
页码:1431 / 1438
页数:8
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