Development and validation of a LC-MS/MS method for the determination of clebopride and its application to a pharmacokinetics study in healthy Chinese volunteers

被引:4
|
作者
Tan, Zhirong [1 ]
Ouyang, Dongsheng [1 ]
Chen, Yao [1 ]
Zhou, Gan [1 ]
Cao, Shan [1 ]
Wang, Yicheng [1 ]
Peng, Xiujuan [1 ]
Zhou, Honghao [1 ]
机构
[1] Cent S Univ, Inst Clin Pharmacol, Pharmacogenet Res Inst, Changsha 410078, Hunan, Peoples R China
关键词
LC-MS/MS; Clebopride; Pharmacokinetics; IONIZATION MASS-SPECTROMETRY; PLASMA;
D O I
10.1016/j.jchromb.2010.06.006
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive and specific liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) method has been developed and validated for the identification and quantification of clebopride in human plasma using itopride as an internal standard. The method involves a simple liquid-liquid extraction. The analytes were separated by isocratic gradient elution on a CAPCELL MG-III C-18 (5 mu m, 150 mm x 2.1 mm i.d.) column and analyzed in multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI) interface using the respective [M+H](+) ions, m/z 373.9 -> m/z184.0 for clebopride, m/z 359.9 -> m/z71.5 for itopride. The method was validated over the concentration range of 69.530-4450.0 pg/ml for clebopride. Within- and between-batch precision (RSD%) was all within 6.83% and accuracy ranged from -8.16 to 1.88%. The LLOQ was 69.530 pg/ml. The extraction recovery was on an average 77% for clebopride. The validated method was used to study the pharmacokinetics profile of clebopride in human plasma after oral administration of clebopride. (C) 2010 Published by Elsevier B.V.
引用
收藏
页码:2072 / 2076
页数:5
相关论文
共 50 条
  • [1] Development and Validation of a LC-MS/MS Method for the Determination of Ilaprazole Enantiomers and Its Application to a Bioequivalence Study in Healthy Chinese Volunteers
    Tan, Zhi-rong
    Wang, Yi-cheng
    Chen, Yao
    Guo, Dong
    Cao, Shan
    Zhang, Wei
    Zhou, Gan
    Zhou, Hong-hao
    Ouyang, Dong-sheng
    CHROMATOGRAPHIA, 2012, 75 (3-4) : 95 - 101
  • [2] Determination of ifenprodil by LC-MS/MS and its application to a pharmacokinetic study in healthy Chinese volunteers
    Yang, Jing
    Lu, Chengtao
    Song, Wei
    Li, Jiankang
    Ding, Yi
    Zhu, Yanrong
    Cao, Jinyi
    Ding, Likun
    Jia, Yanyan
    Wen, Aidong
    ACTA PHARMACEUTICA SINICA B, 2013, 3 (03) : 180 - 184
  • [3] Determination of mildronate by LC-MS/MS and its application to a pharmacokinetic study in healthy Chinese volunteers
    Peng, Ye
    Yang, Jing
    Wang, ZhiRui
    Wang, Jingwen
    Liu, Yu
    Luo, Zhuojing
    Wen, AiDong
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (5-6): : 551 - 556
  • [4] Development and Validation of a LC–MS/MS Method for the Determination of Ilaprazole Enantiomers and Its Application to a Bioequivalence Study in Healthy Chinese Volunteers
    Zhi-rong Tan
    Yi-cheng Wang
    Yao Chen
    Dong Guo
    Shan Cao
    Wei Zhang
    Gan Zhou
    Hong-hao Zhou
    Dong-sheng Ouyang
    Chromatographia, 2012, 75 : 95 - 101
  • [5] A Sensitive and Reliable Method for the Determination of Ezetimibe by LC-MS/MS and its Application to a Pharmacokinetic Study in Healthy Chinese Volunteers
    Li, Yanru
    Tang, Ling
    Wang, Yan
    LATIN AMERICAN JOURNAL OF PHARMACY, 2020, 39 (10): : 1921 - 1926
  • [6] Determination of domperidone in human plasma by LC-MS and its pharmacokinetics in healthy Chinese volunteers
    Wu, MS
    Gao, L
    Cai, XH
    Wang, GJ
    ACTA PHARMACOLOGICA SINICA, 2002, 23 (03) : 285 - 288
  • [7] A Simple and Sensitive LC-MS/MS Method for the Determination of Salbutamol: Application to a Pharmacokinetic Study in healthy Chinese Volunteers
    Wang, Tao
    Wang, Yilin
    Peng, Anzhong
    Tang, Ling
    Wang, Yan
    LATIN AMERICAN JOURNAL OF PHARMACY, 2022, 41 (03): : 537 - 543
  • [8] A simple and sensitive LC-MS/MS method for determination of doxylamine in human plasma and its application in a bioequivalence study in healthy Chinese volunteers
    Fu, Rong
    Sheng, Mang
    Yang, Can
    Liu, Luyao
    Xiong, Wenqiang
    Wang, Xing
    Chen, Xiaodan
    Yu, Zhongjian
    Gao, Yuan
    Tan, Hong
    Xin, Chenlu
    Zhang, Hong
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2022, 220
  • [9] A rapid and sensitive LC-MS/MS method for determination of lercanidipine in human plasma and its application in a bioequivalence study in Chinese healthy volunteers
    Li, Xiaobing
    Shi, Fuguo
    He, Xiaojing
    Jian, Lingyan
    Ding, Li
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2016, 128 : 67 - 72
  • [10] Determination of cefcapene acid by LC-MS and their application to a pharmacokinetic study in healthy Chinese volunteers
    Hong-Fei Duan
    Li Ding
    Xiao-Bing Li
    Lin-Lin Hu
    Ai-Dong Wen
    Ye Leng
    Zhen Liu
    JournalofPharmaceuticalAnalysis, 2013, 3 (02) : 84 - 92