Niosomes based on synthetic cationic lipids for gene delivery: the influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells

被引：44

作者：

Ojeda, E. ^{[1
,2
]}

Puras, G. ^{[1
,2
]}

Agirre, M. ^{[1
,2
]}

Zarate, J. ^{[1
,2
]}

Grijalvo, S. ^{[2
,3
]}

Pons, R. ^{[2
,3
]}

Eritja, R. ^{[2
,3
]}

Martinez-Navarrete, G. ^{[2
,4
]}

Soto-Sanchez, C. ^{[2
,4
]}

Fernandez, E. ^{[2
,4
]}

Pedraz, J. L. ^{[1
,2
]}

机构：

[1] Univ Basque Country, NanoBioCel Grp, Vitoria, Spain

[2] Networking Res Ctr Bioengn Biomat & Nanomed CIBER, Madrid, Spain

[3] IQAC CSIC, Inst Adv Chem Catalonia, Barcelona, Spain

[4] Miguel Hernandez Univ, Neuroprothesis & Neuroengn Res Grp, Elche, Spain

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2015年 / 13卷 / 04期

关键词：

IN-VITRO; MAMMALIAN-CELLS; DNA COMPLEXES; NANOPARTICLES; LIPOPLEXES; THERAPY; EFFICACY; DRUG; VIVO; FORMULATION;

D O I：

10.1039/c4ob02087a

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pK(a), size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.

引用

页码：1068 / 1081

页数：14