Chemical alteration by tooth bleaching of human salivary proteins that infiltrated subsurface enamel lesions -Experimental study with bovine lesion model systems-

被引：8

作者：

Iizuka, Junko ^{[1
,3
]}

Mukai, Yoshiharu ^{[1
,3
]}

Taniguchi, Motoe ^{[2
]}

Mikuni-Takagaki, Yuko ^{[2
]}

Ten Cate, Jacob Martien ^{[4
]}

Teranaka, Toshio ^{[1
,3
]}

机构：

[1] Kanagawa Dent Univ, Grad Sch Dent, Dept Catiol & Restorat Dent, Yokosuka, Kanagawa 2388580, Japan

[2] Kanagawa Dent Univ, Grad Sch Dent, Dept Mol & Cellular Biol Mineralized Tissu, Yokosuka, Kanagawa 2388580, Japan

[3] Kanagawa Dent Univ, Inst Oral Regenerat Med, Yokosuka, Kanagawa 2388580, Japan

[4] Acad Ctr Dent Amsterdam, Dept Prevent Dent, NL-1081 LA Amsterdam, Netherlands

来源：

DENTAL MATERIALS JOURNAL | 2014年 / 33卷 / 05期

基金：

日本学术振兴会;

关键词：

Enamel subsurface lesion; Bleaching; Salivary protein; Remineralization; Statherin; CALCIUM-PHOSPHATE PRECIPITATION; PROLINE-RICH PROTEINS; HUMAN-PAROTID SALIVA; IN-VITRO; LIMITED DEGRADATION; MUCUS GLYCOPROTEINS; CARIOUS LESIONS; FLOW-RATE; STATHERIN; REMINERALIZATION;

D O I：

10.4012/dmj.2014-046

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Salivary macromolecules infiltrate white and brown spot enamel lesions and adsorb onto hydroxyapatite. Calcium-binding salivary proteins such as statherin hinder remineralization of these lesions. We assessed whether bleaching agents can remove salivary components that have infiltrated and bound to experimental subsurface lesions in bovine enamel prepared by immersing specimens in acid and then human saliva. Transversal microradiography showed that such demineralized lesions mimicked incipient carious lesions. Bound proteins to the experimental and untreated control specimens were eluted in a stepwise manner with phosphate-buffered saline, 0.4 M phosphate buffer, and 1 M HCl. SDS-PAGE of dialyzed extracts showed that specific salivary proteins bound to the lesions, while virtually no protein bands were detected if the specimens were bleached. Western blotting showed that even statherin, which was more firmly bound than other proteins, was removed. In-office bleaching agent may be useful in treating enamel lesions for removing proteins bound to these lesions.

引用

页码：663 / 668

页数：6