PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER)

Aminopeptidase N (APN) and cadherin- like proteins have been previously identified as Cry1Ac- binding proteins in Helicoverpa armigera (Hu " bner). In this study, a proteomic approach was used to identify novel Cry1Ac- binding proteins in H. armigera. Brush border membrane vesicles (BBMV) of H. armigera were extracted and separated by two- dimensional gel electrophoresis (2- DE). Cry1Ac- binding proteins were detected using antisera against Cry1Ac. Peptide mass fingerprinting (PMF) was used to identify Cry1Ac- binding proteins. In total, four proteins were identified as candidate Cry1Ac- binding proteins in H. armigera: vacuolar ATP synthase (V- ATPase) subunit B, actin, heat shock cognate protein (HSCP), and a novel protein. (C) 2009 Wiley Periodicals, Inc.