Picosecond protein response to the chromophore isomerization of photoactive yellow protein: Selective observation of tyrosine and tryptophan residues by time-resolved ultraviolet resonance raman spectroscopy

被引：21
|
作者：
Mizuno, Misao
Hamada, Norio
Tokunaga, Fumio
Mizutani, Yasuhisa
机构：
[1] Osaka Univ, Grad Sch Sci, Dept Chem, Osaka 5600043, Japan
[2] Osaka Univ, Venture Business Lab, JST CREST, Osaka 5650871, Japan
[3] Osaka Univ, Grad Sch Sci, Dept Earth & Space Sci, Osaka 5600043, Japan
来源：
JOURNAL OF PHYSICAL CHEMISTRY B | 2007年 / 111卷 / 23期
关键词：
D O I：
10.1021/jp072939d
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Picosecond time-resolved ultraviolet resonance Raman (UVRR) spectra of photoactive yellow protein (PYP) were measured. UVRR bands attributed to the vibration of tyrosine and tryptophan residues showed a spectral change upon photoreaction. It was found that the hydrogen-bond strength between the chromophore and Y42 increases in the pG* state. The ultrafast change in the tryptophan band revealed that a photoinduced structural change of the chromophore had propagated to the W119 region, located 12 angstrom from the chromophore, within picoseconds.
引用
收藏
页码：6293 / 6296
页数：4
相关论文
共 33 条
  • [1] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin: Primary Protein Response to the Photoisomerization of Retinal
    Mizuno, Misao
    Shibata, Mikihiro
    Yamada, Junya
    Kandori, Hideki
    Mizutani, Yasuhisa
    JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (35): : 12121 - 12128
  • [2] Time-resolved FTIR spectroscopy of photoactive yellow protein.
    Kelemen, L
    Hendriks, J
    Hellingwerf, KJ
    Xie, AH
    BIOPHYSICAL JOURNAL, 2000, 78 (01) : 476A - 476A
  • [3] Structural changes during the photocycle of photoactive yellow protein monitored by ultraviolet resonance Raman spectra of tyrosine and tryptophan
    El-Mashtoly, SF
    Yamauchi, S
    Kumauchi, M
    Hamada, N
    Tokunaga, F
    Unno, M
    JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (49): : 23666 - 23673
  • [4] Ultrafast Structural Evolution of Photoactive Yellow Protein Chromophore Revealed by Ultraviolet Resonance Femtosecond Stimulated Raman Spectroscopy
    Kuramochi, Hikaru
    Takeuchi, Satoshi
    Tahara, Tahei
    JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2012, 3 (15): : 2025 - 2029
  • [5] Time-resolved resonance Raman structural studies of the pB′ intermediate in the photocycle of photoactive yellow protein
    Pan, DH
    Philip, A
    Hoff, WD
    Mathies, RA
    BIOPHYSICAL JOURNAL, 2004, 86 (04) : 2374 - 2382
  • [6] Ultrafast protein dynamics of hemoglobin as studied by picosecond time-resolved resonance Raman spectroscopy
    Mizutani, Yasuhisa
    Nagai, Masako
    CHEMICAL PHYSICS, 2012, 396 : 45 - 52
  • [7] Dynamics of protein and chromophore structural changes in the photocycle of photoactive yellow protein monitored by time-resolved optical rotatory dispersion
    Chen, EF
    Gensch, T
    Gross, AB
    Hendriks, J
    Hellingwerf, KJ
    Kliger, DS
    BIOCHEMISTRY, 2003, 42 (07) : 2062 - 2071
  • [8] Time-resolved single tryptophan fluorescence in photoactive yellow protein monitors changes in the chromophore structure during the photocycle via energy transfer
    Otto, H
    Hoersch, D
    Meyer, TE
    Cusanovich, MA
    Heyn, MP
    BIOCHEMISTRY, 2005, 44 (51) : 16804 - 16816
  • [9] Skeletal Structure of the Chromophore of Photoactive Yellow Protein in the Excited State Investigated by Ultraviolet Femtosecond Stimulated Raman Spectroscopy
    Kuramochi, Hikaru
    Takeuchi, Satoshi
    Kamikubo, Hironari
    Kataoka, Mikio
    Tahara, Tahei
    JOURNAL OF PHYSICAL CHEMISTRY B, 2021, 125 (23): : 6154 - 6161
  • [10] PICOSECOND TIME-RESOLVED RESONANCE RAMAN-SPECTROSCOPY OF THE INITIAL TRANS TO CIS ISOMERIZATION IN THE BACTERIORHODOPSIN PHOTOCYCLE
    ATKINSON, GH
    BRACK, TL
    BLANCHARD, D
    RUMBLES, G
    CHEMICAL PHYSICS, 1989, 131 (01) : 1 - 15
1234