Developing a MAb Aggregate Removal Step by High Throughput Process Development

被引：0

作者：

Rodrigo, Gustav ^{[1
]}

Nilsson-Valimaa, Kristina ^{[1
]}

机构：

[1] GE Healthcare, Uppsala, Sweden

来源：

BIOPHARM INTERNATIONAL | 2010年 / 23卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This article presents a case study illustrating the benefits of using a high throughput process development (HTPD) approach for choice of media and optimization of the polishing step of a monoclonal antibody in a two-step purification process. The most commonly used format for HTPD is the 96-well plate to facilitate rapid screening of chromatographic parameters. The use of the 96-well plate format enabled the efficient screening of three different media and optimization of chromatographic parameters to maximize yield at the desired purity level.

引用

页码：46 / 51

页数：6

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