Dissolving Microneedle Patches for Transdermal Insulin Delivery in Diabetic Mice: Potential for Clinical Applications

被引:59
|
作者
Chen, Chih-Hao [1 ,2 ]
Shyu, Victor Bong-Hang [2 ]
Chen, Chien-Tzung [2 ,3 ]
机构
[1] Chang Gung Univ, Dept Chem & Mat Engn, Taoyuan 333, Taiwan
[2] Chang Gung Univ, Chang Gung Mem Hosp Linkou, Dept Plast & Reconstruct Surg, Craniofacial Res Ctr,Coll Med, Taoyuan 333, Taiwan
[3] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp Keelung, Dept Plast & Reconstruct Surg, Keelung 204, Taiwan
关键词
microneedle; polymer; transdermal; insulin; diabetes mellitus; DRUG-DELIVERY; SKIN THICKNESS; POLYMER MICRONEEDLES; HUMAN VOLUNTEERS; PENETRATION; FORMULATION; FABRICATION; CHALLENGES; TRANSPORT; ARRAYS;
D O I
10.3390/ma11091625
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
In this study, dissolving polymeric microneedle (MN) patches composed of gelatin and sodium carboxymethyl cellulose (CMC) were used to localize insulin. Their in vitro skin insertion capabilities were determined using tissue-marking dye to stain the skin after patches removal. Scanning electron microscopy (SEM) was used to determine changes in the MNs over time, and optical coherence tomography (OCT) was used to monitor their real-time penetration depth. Confocal microscopy images revealed that rhodamine 6G gradually diffuses from the puncture sites to deeper dermal tissue. Using an in vivo imaging system (IVIS), skin areas that received FITC-insulin-loaded MNs were found to present strong fluorescent signals that greatly decreased 1 h after application. Results show that dissolving MNs rapidly release FITC-insulin, and it then gradually diffuses into the skin. This study verifies that using a gelatin/CMC MN patch for insulin delivery achieves satisfactory relative bioavailability compared to a traditional hypodermic injection and can be a promising delivery device for poorly permeable protein drugs such as those used to treat diabetes. Insertion tests on human cadaveric skin demonstrate that dissolving MNs could serve as efficient devices for transdermal drug delivery in clinical practice and that the volar aspect of forearm skin is the ideal location for their applications.
引用
收藏
页数:10
相关论文
共 50 条
  • [1] Formulation of two-layer dissolving polymeric microneedle patches for insulin transdermal delivery in diabetic mice
    Lee, I-Chi
    Lin, Wei-Ming
    Shu, Jwu-Ching
    Tsai, Shau-Wei
    Chen, Chih-Hao
    Tsai, Meng-Tsan
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2017, 105 (01) : 84 - 93
  • [2] Dissolving polymer microneedle patches for rapid and efficient transdermal delivery of insulin to diabetic rats
    Ling, Ming-Hung
    Chen, Mei-Chin
    ACTA BIOMATERIALIA, 2013, 9 (11) : 8952 - 8961
  • [3] Smart microneedle patches for rapid, and painless transdermal insulin delivery
    Wang, Yuanpeng
    Wang, Haozheng
    Zhu, X. X.
    Guan, Ying
    Zhang, Yongjun
    JOURNAL OF MATERIALS CHEMISTRY B, 2020, 8 (40) : 9335 - 9342
  • [4] Rapidly Dissolving Microneedle Patches for Transdermal Iron Replenishment Therapy
    Maurya, Abhijeet
    Nanjappa, Shivakumar H.
    Honnavar, Swati
    Salwa, M.
    Murthy, S. Narasimha
    JOURNAL OF PHARMACEUTICAL SCIENCES, 2018, 107 (06) : 1642 - 1647
  • [5] Nanosuspension-Loaded Dissolving Microneedle Patches for Enhanced Transdermal Delivery of a Highly Lipophilic Cannabidiol
    Cheng, Aguo
    Zhang, Suohui
    Meng, Fanda
    Xing, Mengzhen
    Liu, Han
    Yang, Guozhong
    Gao, Yunhua
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2024, 19 : 4061 - 4079
  • [6] Phase-Transition Microneedle Patches for Efficient and Accurate Transdermal Delivery of Insulin
    Yang, Sixing
    Wu, Fei
    Liu, Jianguo
    Fan, Guorong
    Welsh, William
    Zhu, Hua
    Jin, Tuo
    ADVANCED FUNCTIONAL MATERIALS, 2015, 25 (29) : 4633 - 4641
  • [7] Dissolving microneedle rollers for rapid transdermal drug delivery
    Xiao Peng Zhang
    Bao Li Zhang
    Bo Zhi Chen
    Ze Qiang Zhao
    Wen Min Fei
    Yong Cui
    Xin Dong Guo
    Drug Delivery and Translational Research, 2022, 12 : 459 - 471
  • [8] Dissolving Microneedle Arrays for Transdermal Delivery of Amphiphilic Vaccines
    An, Myunggi
    Liu, Haipeng
    SMALL, 2017, 13 (26)
  • [9] Dissolving microneedle rollers for rapid transdermal drug delivery
    Zhang, Xiao Peng
    Zhang, Bao Li
    Chen, Bo Zhi
    Zhao, Ze Qiang
    Fei, Wen Min
    Cui, Yong
    Guo, Xin Dong
    DRUG DELIVERY AND TRANSLATIONAL RESEARCH, 2022, 12 (02) : 459 - 471
  • [10] Dissolving microneedle patch for transdermal delivery of perindopril erbumine
    Altaf, Zunaira
    Ahmad, Zulcaif
    Mahmood, Asif
    Shchinar, Saniia
    Latif, Riffat
    INFLAMMOPHARMACOLOGY, 2025, 33 (03) : 1381 - 1391