Evaluation of genetic diversity of Ralstonia solanacearum causing bacterial wilt of ginger using REP-PCR and PCR-RFLP

被引：0
|
作者：
Kumar, A [1 ]
Sarma, YR [1 ]
Anandaraj, M [1 ]
机构：
[1] Indian Inst Spices Res, Div Crop Protect, Kolkata 673012, W Bengal, India
来源：
CURRENT SCIENCE | 2004年 / 87卷 / 11期
关键词：
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Thirty-three strains of Ralstonia solanacearum Yabuuchi (Smith) isolated from. ginger, paprika, chilli, tomato, Chromolaena and potato from Kerala, Karnataka, West Bengal and Assam in India, were phenotypically and genotypically characterized. Phenotypic characterization for biovar revealed the predominance of biovar 3 in India. Molecular analysis by REP-PCR, ITS-PCR and RFLP-PCR classified the strains into three clusters at 70% similarity, where ginger strains are grouped in Clusters I and II. Strains from potato (biovar 2) clustered in the III cluster. Molecular analysis also revealed that ginger strains isolated from different locations during different years had 100% similarity according to Dice's coefficient. The analysis further revealed that the genetic diversity of Ralstonia is very low within ginger, confirming that the pathogen population is of clonal lineage and is distributed through 'rhizome transmission' of the inoculum between locations and also between seasons within the locality.
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页码：1555 / 1561
页数：7
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