Modulation of HOXA10 and other markers of endometrial receptivity by age and human chorionic gonadotropin in an endometrial explant model

Objective: To characterize the endometrial response to human chorionic gonadotropin (hCG), as influenced by uterine age, using endometrial receptivity markers including HOXA10, vascular endothelial growth factor (VEGF), and glycodelin in an endometrial explant culture system. Design: In vitro molecular biology research. Setting: Academic infertility clinic and molecular biology laboratory. Patient(s): Fourteen prospective recipients of egg donation (mean age, 44 +/- 8 years). Intervention(s): Subjects received cyclical estrogen (E-2) and progesterone (P-4) and underwent,in endometrial biopsy on day 7 of P-4. Endometrial biopsy samples were cut into 1-mm(3) pieces and cultured in Dulbecco's modified Eagle's medium/Ham's F-12 with E-2 and P-4, Without (control) or with hCG (400, 4000, and 40,000 mlU/mL) oil Millicell-CM inserts for 24 hours. Main Outcome Measure(S): Explant viability was assessed using immunohistochemistry (IHC). Semiquantitative polymerase chain reaction was performed to evaluate relative gene expression via mRNA levels of HOXA10, VEGF, and glycodelin. Result(s): Explant viability was confirmed oil IHC by histology and Ki-67 staining, a marker of proliferation. HOXA10, VEGF, and glycodelin gene expression increased at all concentrations of hCG over those of controls. HOXA10 gene expression was inversely correlated with age (-0.08- +/- 0.03-fold decrease in gene expression/year of age). Conclusion(s): The endometrial explant culture system is a promising model for the study of endometrial response as it maintains interactions among the stroma, glands, and epithelium. HOXA 10, VEGF and glycodelin all demonstrated increased gene expression in response to increasing hCG concentrations, Supporting the role of hCG as a candidate protein for blastocyst-endometrial communication. Statistically significant associations between age and expression of HOXA 10 provide novel evidence that uterine age may play a role in endometrial response on It Molecular level. (Fertil Steril (R) 2010;93:1255-9. (C) 2010 by American Society for Reproductive Medicine.)