Nanoscale analysis of supported lipid bilayers using atomic force microscopy

被引:119
|
作者
El Kirat, Karim [1 ]
Morandat, Sandrine [2 ]
Dufrene, Yves F. [3 ]
机构
[1] Univ Technol Compiegne, CNRS, Lab Biomecan & Bioingn, UMR 6600, F-60205 Compiegne, France
[2] Univ Technol Compiegne, CNRS, Lab Genie Enzymat & Cellulaire, UMR 6022, F-60205 Compiegne, France
[3] Catholic Univ Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium
来源
关键词
Atomic force microscopy; Biomembranes; Nanoscale organization; Real-time imaging; Supported lipid bilayers; PHOSPHOLIPASE A(2) HYDROLYSIS; GEL-PHASE DOMAINS; MEMBRANE-PROTEIN INSERTION; SCALE SURFACE-PROPERTIES; HIGH-RESOLUTION IMAGES; GPI-ANCHORED PROTEINS; LIQUID-ORDERED PHASE; AIR-WATER-INTERFACE; SOLID-STATE NMR; IN-SITU;
D O I
10.1016/j.bbamem.2009.07.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During the past 15 years, atomic force microscopy (AFM) has opened new opportunities for imaging supported lipid bilayers (SLBs) on the nanoscale. AFM offers a means to visualize the nanoscale structure of SLBs in physiological conditions. A unique feature of AFM is its ability to monitor dynamic events, like bilayer alteration, remodelling or digestion, upon incubation with various external agents such as drugs, detergents, proteins, peptides, nanoparticles, and solvents. Here, we survey recent progress made in the area. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:750 / 765
页数:16
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