Metabolomics Based on UHPLC-Orbitrap-MS and Global Natural Product Social Molecular Networking Reveals Effects of Time Scale and Environment of Storage on the Metabolites and Taste Quality of Raw Pu-erh Tea

被引：101

作者：

Xu, Shanshan ^{[1
]}

Wang, Jing-Jing ^{[1
]}

Wei, Yuming ^{[1
]}

Deng, Wei-Wei ^{[1
]}

Wan, Xiaochun ^{[1
]}

Bao, Guan-Hu ^{[1
]}

Xie, Zhongwen ^{[1
]}

Ling, Tie-Jun ^{[1
]}

Ning, Jingming ^{[1
]}

机构：

[1] Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, 130 West Changjiang Rd, Hefei 230036, Anhui, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 43期

基金：

中国国家自然科学基金;

关键词：

raw Pu-erh tea; storage; environment; time scale; metabolomics; UHPLC-Orbitrap-MS; GNPS; molecular networking; SIRIUS; electronic tongue; MASS-SPECTROMETRY; CONSTITUENTS; FLAVAN-3-OLS; RING;

D O I：

10.1021/acs.jafc.9b05314

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Raw Pu-erh tea (RPT) needs ageing before drinking. However, the influence from environment and time of storage on chemical profile and flavor of RPT is unclear. In this study, the RPTs stored in wet-hot or dry-cold environment for 1-9 years were assessed using metabolomics based on UHPLC-Orbitrap-MS and global natural product social (GNPS) feature-based molecular networking as well as electronic tongue measurement. The results exhibited that the chemical profiles of RPTs were similar at an early stage but started to differentiate from each other at the 5th and the 7th year in wet-hot and dry-cold environments. The discriminating features including N-ethyl-2-pyrrolidinone-substituted flavan-3-ols (flavoalkaloids), unsaturated fatty acids, lysophosphatidylcholines, flavan-3-ols, amino acids, and flavonol-O-glycosides among the three chemical profiles were discovered and analyzed by means of multivariate statistics, GNPS multilibraries matching, and SIRIUS calculation. The metabolomic data were consistent with the results obtained through electronic tongue measurement.

引用

页码：12084 / 12093

页数：10