ISOLATION AND CHARACTERIZATION OF MICROSATELLITE LOCI IN THE COMMON MILKWEED, ASCLEPIAS SYRIACA (APOCYNACEAE)

被引:11
|
作者
Kabat, Susan M. [1 ]
Dick, Christopher W. [2 ,3 ,4 ]
Hunter, Mark D. [2 ]
机构
[1] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Herbarium, Ann Arbor, MI 48109 USA
[4] Smithsonian Trop Res Inst, Balboa, Ancon, Panama
基金
美国国家科学基金会;
关键词
Apocynaceae; Asclepias syriaca; microsatellite; milkweed; HERBIVORE; SOUTHWARD;
D O I
10.3732/ajb.1000064
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Premise of the study: Microsatellite primers were developed for the common milkweed, Asclepias syriaca L., to assist in genet identification and the analysis of spatial genetic structure. Methods and Results: Using an enrichment cloning protocol, eight microsatellite loci were isolated and characterized in a Michigan population of A. syriaca. The primers amplified di- and trinucleotide repeats with 4-13 alleles per locus. Conclusions: The primers will be useful for studies of clonality and gene flow in natural populations.
引用
收藏
页码:E37 / E38
页数:2
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