Stearoyl-Coenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins

被引:165
|
作者
Mele, M. [1 ]
Conte, G.
Castiglioni, B.
Chessa, S.
Macciotta, N. P. P.
Serra, A.
Buccioni, A.
Pagnacco, G.
Secchiari, P.
机构
[1] Univ Pisa, Dipartimento Agron & Gest Agroecosistema, I-56100 Pisa, Italy
[2] CNR, Ist Biol & Biotechnol Agr, I-20133 Milan, Italy
[3] Univ Milan, Dipartimento Sci & Tecnol Vet Sicurezza Alimentar, I-20122 Milan, Italy
[4] Univ Sassari, Dipartimento Sci Zootecn, I-07100 Sassari, Italy
[5] Univ Florence, Dipartimento Sci Zootecn, Florence, Italy
关键词
stearoyl-CoA desaturase polymorphism; conjugated linoleic acid; monounsaturated fatty acid;
D O I
10.3168/jds.2006-617
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Milk fatty acid composition is a parameter of great interest for evaluation of nutritional quality of milk. Stearoyl-CoA desaturase (SCD) is a key enzyme in mammary lipid metabolism because it is able to add a double bond in the cis Delta(9)- position in a large spectrum of medium- and long-chain fatty acids. A polymorphism with 2 alleles (A and V) in the fifth exon of the SCD gene has been reported. The effect of SCD genotype on individual milk fatty acid composition and on cis-9 unsaturated/saturated fatty acid ratios of 297 Holstein Italian Friesian cows was investigated in this paper. The SCD genotypes were determined by using a single strand conformation polymorphism method. Relative frequencies of SCD genotypes were 27, 60, and 13% for AA, AV, and VV, respectively. Milk of AA cows had a greater content of cis-9 C18:1 and total monounsaturated fatty acids and a higher C14:1/C14 ratio than did milk of VV cows. The relative contribution of SCD genotype to variation of monounsaturated fatty acids, cis-9 C18:1, and cis-9 C14:1 was 5, 4, and 7.7%, respectively. No significant differences were detected between SCD genotypes in the milk content of cis-9, trans-11 C18:2. Results of the present work provide some indication of an association between SCD locus and the fatty acid profile in the examined sample of Italian Holsteins, thus suggesting a possible role of this gene in the genetic variation of milk nutritional properties.
引用
收藏
页码:4458 / 4465
页数:8
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