A novel technology for packing and unpacking pilot and production scale columns

被引：13
|
作者：
Hofmann, M [1 ]
机构：
[1] Euroflow UK Ltd, Stroud GL5 2QN, Glos, England
来源：
JOURNAL OF CHROMATOGRAPHY A | 1998年 / 796卷 / 01期
关键词：
preparative chromatography; packing of columns;
D O I：
10.1016/S0021-9673(97)00727-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method of packing and unpacking large scale chromatography columns is described. This involves use of a 3-position valve that can inject chromatography media into a closed column thereby packing it. This same valve in another state is then used to unpack the column. Heights equivalent to a theoretical plate and asymmetry factors of packs on columns from 280 to 800 mm diameter are discussed. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：75 / 80
页数：6
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