Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213

被引：20
|
作者：
Li, Jinlong [1 ,2 ]
Shen, Weijia [1 ,2 ]
Fan, Guangsen [1 ,3 ]
Li, Xiuting [1 ,2 ]
机构：
[1] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Fucheng Rd, Beijing 100048, Peoples R China
[2] Beijing Higher Inst, Engn Res Ctr Food Addit & Ingredients, Beijing 100048, Peoples R China
[3] Beijing Key Lab Flavor Chem, Beijing 100048, Peoples R China
来源：
3 BIOTECH | 2018年 / 8卷 / 09期
基金：
中国国家自然科学基金;
关键词：
B; pyrrocinia; Lipase; Screening; Purification; Characterization; SOLVENT-TOLERANT LIPASE; MICROBIAL LIPASES; INDUSTRIAL APPLICATIONS; CANDIDA-ANTARCTICA; BACTERIAL LIPASES; FUNGAL LIPASES; TRANSESTERIFICATION; BIOTECHNOLOGY;
D O I：
10.1007/s13205-018-1414-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of this lipase was pH 8.0 and 50 degrees C, respectively. The lipase retained > 85% activity in isopropanol and acetone at 30 degrees C for 10 mM but the activity was reduced to 10.6% in n-hexane. Mg2+, Al3+, Mn2+, and Fe3+ enhanced lipase activity at both 1 mM and 5 mM concentrations. p-NPP, a long-chain acyl group 4-NP ester, appeared to be a good substrate candidate.
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页数：12
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