Requirement of the spindle checkpoint for proper chromosome segregation in budding yeast meiosis

被引:181
|
作者
Shonn, MA
McCarroll, R
Murray, AW
机构
[1] Univ Calif San Francisco, Dept Biochem, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA
[3] Cereon Genom LLC, Cambridge, MA 02139 USA
关键词
D O I
10.1126/science.289.5477.300
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The spindle checkpoint was characterized in meiosis of budding yeast. In the absence of the checkpoint, the frequency of meiosis I missegregation increased with increasing chromosome Length, reaching 19% for the longest chromosome. Meiosis I nondisjunction in spindle checkpoint mutants could be prevented by delaying the onset of anaphase. In a recombination-defective mutant (spo11 Delta), the checkpoint delays the biochemical events of anaphase I, suggesting that chromosomes that are attached to microtubules but are not under tension can activate the spindle checkpoint. Spindle checkpoint mutants reduce the accuracy of chromosome segregation in meiosis I much more than that in meiosis II, suggesting that checkpoint defects may contribute to Down syndrome.
引用
收藏
页码:300 / 303
页数:4
相关论文
共 50 条
  • [1] The spindle checkpoint and chromosome segregation in meiosis
    Gorbsky, Gary J.
    [J]. FEBS JOURNAL, 2015, 282 (13) : 2458 - 2474
  • [2] Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast
    Gillett, ES
    Espelin, CW
    Sorger, PK
    [J]. JOURNAL OF CELL BIOLOGY, 2004, 164 (04): : 535 - 546
  • [3] The spindle assembly checkpoint in budding yeast
    Straight, AF
    Murray, AW
    [J]. CELL CYCLE CONTROL, 1997, 283 : 425 - 440
  • [4] Spindle checkpoint control in budding yeast
    Toh-e, A
    Asakawa, K
    Yoshida, S
    [J]. JOURNAL OF MICROBIOLOGY, 2001, 39 (01) : 1 - 10
  • [5] The spindle assembly checkpoint: Preventing chromosome mis-segregation during mitosis and meiosis
    Malmanche, Nicolas
    Maia, Andre
    Sunkel, Claudio E.
    [J]. FEBS LETTERS, 2006, 580 (12): : 2888 - 2895
  • [6] Regulation of the spindle position checkpoint in budding yeast
    Pereira, G.
    Schiebel, E.
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2005, 141 (03): : S324 - S324
  • [7] Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast
    Matsuhara, Hirotada
    Yamamoto, Ayumu
    [J]. GENES TO CELLS, 2016, 21 (01) : 65 - 87
  • [8] Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption
    Hardwick, KG
    Weiss, E
    Luca, FC
    Winey, M
    Murray, AW
    [J]. SCIENCE, 1996, 273 (5277) : 953 - 956
  • [9] The spindle position checkpoint in budding yeast: the motherly care of MEN
    Simonetta Piatti
    Marianna Venturetti
    Elena Chiroli
    Roberta Fraschini
    [J]. Cell Division, 1
  • [10] Budding yeast Cdc20: A target of the spindle checkpoint
    Hwang, LH
    Lau, LF
    Smith, DL
    Mistrot, CA
    Hardwick, KG
    Hwang, ES
    Amon, A
    Murray, AW
    [J]. SCIENCE, 1998, 279 (5353) : 1041 - 1044