Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy

被引：13
|
作者：
Tserevelakis, G. J. [1 ,3 ]
Filippidis, G. [1 ]
Krmpot, A. J. [1 ,4 ]
Vlachos, M. [2 ]
Fotakis, C. [1 ,3 ]
Tavernarakis, N. [2 ]
机构：
[1] Fdn Res & Technol Hellas, Inst Elect Struct & Laser, Iraklion 71110, Crete, Greece
[2] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion 71110, Crete, Greece
[3] Univ Crete, Dept Phys, Khania, Greece
[4] Univ Belgrade, Inst Phys, Belgrade 11080, Serbia
来源：
MICRON | 2010年 / 41卷 / 05期
关键词：
THG; Imaging; Caenorhabditis elegans; Embryogenesis; MORPHOGENETIC MOVEMENTS; TISSUES; EMBRYOS; CELLS;
D O I：
10.1016/j.micron.2010.02.006
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex developmental process of C. elegans embryogenesis. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：444 / 447
页数：4
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