Chlorophyll Inhibits the Digestion of Soybean Oil in Simulated Human Gastrointestinal System

被引:7
|
作者
Wang, Xiao [1 ,2 ,3 ]
Li, Yuanyuan [1 ,2 ,3 ,4 ]
Shen, Suxia [1 ,2 ,3 ]
Yang, Zhaotian [1 ,2 ,3 ]
Zhang, Haifeng [5 ]
Zhang, Yan [1 ,2 ,3 ]
机构
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
[2] Minist Sci & Technol, Natl Engn Res Ctr Fruits & Vegetables Proc, Beijing 100083, Peoples R China
[3] Minist Agr & Rural Affairs, Key Lab Fruits & Vegetables Proc, Beijing 100083, Peoples R China
[4] Acad Agr Planning & Engn, Key Lab Agroprod Postharvest Handing, Minist Agr & Rural Affairs, Beijing 100125, Peoples R China
[5] Precis Nutr Shenzhen Technol Co Ltd, Shenzhen 518083, Peoples R China
基金
中国国家自然科学基金;
关键词
chlorophyll; pheophytin; gastrointestinal digestion; pancreatic lipase; BINDING; EXTRACTS; FAT; ANTHOCYANINS; FLUORESCENCE; RESVERATROL; QUERCETIN; BEHAVIOR; PROTEIN; OBESITY;
D O I
10.3390/nu14091749
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Nowadays, much available processed and highly palatable food such as cream products and fried and convenient food, which usually showed a high energy density, had caused an increase in the intake of dietary lipids, further leading to significant growth in the prevalence of obesity. Chlorophyll, widespread in fruits and vegetables, was proven to have beneficial effects on alleviating obesity. This study investigated the effects of chlorophyll on the digestive characteristics of lipids under in vitro simulated adult and infant gastrointestinal systems. Chlorophyll decreased the release rate of free fatty acid (FFA) during in vitro adult and infant intestinal digestion by 69.2% and 60.0%, respectively. Meanwhile, after gastrointestinal digestion, chlorophyll changed the FFA composition of soybean oil emulsion and increased the particle size of oil droplets. Interestingly, with the addition of chlorophyll, the activity of pancreatic lipase was inhibited during digestion, which may be related to pheophytin (a derivative of chlorophyll after gastric digestion). Therefore, the results obtained from isothermal titration calorimetry and molecular docking further elucidated that pheophytin could bind to pancreatic lipase with a strong affinity of (4.38 +/- 0.76) x 10(7) M-1 (K-a), while the binding site was amino acid residue Trp253. The investigation not only explained why chlorophyll inhibited digestive enzyme activity to reduce lipids digestion but also provided exciting opportunities for developing novel chlorophyll-based healthy products for dietary application in preventing obesity.
引用
收藏
页数:15
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