Proteomic Analysis of Fear Memory Related Proteins in Mice

被引：0

作者：

Zheng Jun-Fang ^{[1
]}

Liu Hua ^{[1
]}

Xiong Ying ^{[1
]}

Wang Xiao-Zhu ^{[1
]}

He Jun-Qi ^{[1
]}

机构：

[1] Capital Med Univ, Dept Biochem & Mol Biol, Beijing 100069, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2010年 / 31卷 / 04期

关键词：

Fear conditioning; Hippocampus; Proteomics; Mass spectrometry; 2'; 3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE; TRANSGENIC MICE; SYNAPTIC FUNCTION; SPATIAL MEMORY; GROWTH-FACTOR; MOUSE MODEL; EXPRESSION; PLASTICITY; NEURONS; TASK;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To investigate proteins related with fear and emotional memory, hippocampal proteins from CD1 and C57BL/6J mice after fear conditioning experiment were extracted and subjected to 2D gel electrophoresis. Protein quantification was analyzed and the differentially expressed spots were identified using mass spectrometry and MASCOT database searching. Hippocampal proteins from CD1 and C57BL/6J mice after fear conditioning experiment were significantly different. Twenty-nine proteins(Thirty-one spots) are closely related with fear memory formation. Expression levels of 7 spots downregulated significantly; expression levels of 24 spots increased significantly. These identified proteins could be devided into six categories according to their functions: (1) energy metabolism and mitochondrion function; (2) neurogenesis; (3) signal transduction; (4) cytoskeleton; (5) amino acid metabolism and protein degradation; (6) chaperone. These proteins improved insights into the fear mechanism and provided new targets for the study and treatment of cognitive diseases.

引用

页码：736 / 741

页数：6

共 45 条

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