A novel methodology for imaging new bone formation around bioceramic bone substitutes

被引:8
|
作者
Gorustovich, Alejandro A.
Sivak, Matias G.
Guglielmotti, Maria B.
机构
[1] Natl Atom Energy Commiss, CNEA Reg Noroeste, Res Lab, Salta, Argentina
[2] Consejo Nacl Invest Cient & Tecn, Natl Res Council, RA-1033 Buenos Aires, DF, Argentina
[3] Univ Buenos Aires, Sch Dent, Dept Oral Pathol, Buenos Aires, DF, Argentina
关键词
confocal laser scanning microscopy; bone; bioceramics;
D O I
10.1002/jbm.a.31071
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this study, Frost's bulk-staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:443 / 445
页数:3
相关论文
共 50 条
  • [1] Bone substitutes and bone formation
    Stutzle, H
    Hallfeldt, K
    Mandelkow, H
    Kessler, S
    Schweiberer, L
    ORTHOPADE, 1998, 27 (02): : 118 - 125
  • [2] Bioceramic bone graft substitutes: Influence of porosity and chemistry
    Hing, KA
    INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY, 2005, 2 (03) : 184 - 199
  • [3] Guiding bone regeneration with a novel biodegradable polymeric membrane and bioceramic bone grafts around dental implants
    Li, L.
    Bao, C. Y.
    Ou, G. M.
    Chen, W. C.
    Zhang, X. J.
    Yang, D. J.
    Wang, Q.
    Sun, L. Y.
    Xiong, C. D.
    BIOCERAMICS, VOL 19, PTS 1 AND 2, 2007, 330-332 : 1417 - +
  • [4] A New Multilayered Composite Bioceramic for Bone Graft
    Arias, Jose I.
    Neira-Carrillo, Andronico
    Yazdani-Pedram, Mehrad
    Fernandez, Maria S.
    Arias, Jose L.
    STRUCTURE-PROPERTY RELATIONSHIPS IN BIOMINERALIZED AND BIOMIMETIC COMPOSITES, 2009, 1187 : 169 - +
  • [5] Novel microinjector for carrying bone substitutes for bone regeneration in periodontal diseases
    Tsai, Hsiao-Cheng
    Li, Yi-Chen
    Young, Tai-Horng
    Chen, Min-Huey
    JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION, 2016, 115 (01) : 45 - 50
  • [6] Fabrication of a novel bioceramic membrane for guided bone regeneration
    Toda, Manami
    Oshiro, Kazunobu
    Fujimori, Hirotaka
    Yamamoto, Setsuo
    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 2006, 114 (1334) : 799 - 801
  • [7] In-vitro testing of biofilm formation on infected bone grafts and bone substitutes
    Clauss, M.
    Bohner, M.
    Borens, O.
    Trampuz, A.
    Ilchmann, T.
    SWISS MEDICAL WEEKLY, 2010, 140 (23-24) : 41S - 41S
  • [8] The effect of synthetic bone graft substitutes on bone formation in rabbit calvarial defects
    Nikola Saulacic
    Masako Fujioka-Kobayashi
    Yasushi Kimura
    Ava Insa Bracher
    Claudio Zihlmann
    Niklaus P. Lang
    Journal of Materials Science: Materials in Medicine, 2021, 32
  • [9] The effect of synthetic bone graft substitutes on bone formation in rabbit calvarial defects
    Saulacic, Nikola
    Fujioka-Kobayashi, Masako
    Kimura, Yasushi
    Bracher, Ava Insa
    Zihlmann, Claudio
    Lang, Niklaus P.
    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2021, 32 (01)
  • [10] Design and study of in vivo bone formation characteristics of biodegradable bioceramic
    Wang, Su
    Huang, Zhili
    Liu, Linlin
    Shi, Zhang'ao
    Liu, Juncai
    Li, Zhong
    Hao, Yongqiang
    MATERIALS & DESIGN, 2021, 212