irCLIP platform for efficient characterization of protein-RNA interactions

被引：0

作者：

Zarnegar, Brian J. ^{[1
]}

Flynn, Ryan A. ^{[1
,2
]}

Shen, Ying ^{[1
]}

Do, Brian T. ^{[1
,2
]}

Chang, Howard Y. ^{[1
,2
]}

Khavari, Paul A. ^{[1
,3
]}

机构：

[1] Stanford Univ, Sch Med, Program Epithelial Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Ctr Personal Dynam Regulomes, Stanford, CA 94305 USA

[3] Palo Alto Healthcare Syst, Vet Affairs, Palo Alto, CA 94304 USA

来源：

NATURE METHODS | 2016年 / 13卷 / 06期

关键词：

BINDING PROTEIN; HITS-CLIP; NUCLEOTIDE RESOLUTION; WIDE ANALYSIS; TRANSCRIPTOME; SITES; CELLS; ARGONAUTE; INSIGHTS;

D O I：

10.1038/NMETH.3840

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.

引用

页码：489 / +

页数：7

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