Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films

被引：29
|
作者：
Rufato, Kessily B. [2 ]
Souza, Paulo R. [2 ]
de Oliveira, Ariel C. [1 ,2 ]
Berton, Sharise B. R. [1 ,2 ]
Sabino, Roberta M. [4 ,5 ]
Muniz, Edvani C. [2 ,6 ]
Popat, Ketul C. [4 ,5 ]
Radovanovic, Eduardo [2 ]
Kipper, Matt J. [3 ,4 ,5 ]
Martins, Alessandro F. [1 ,2 ,3 ]
机构：
[1] Fed Univ Technol Parana UTFPR Apucarana, Lab Mat Macromol & Composites, BR-86812460 Apucarana, PR, Brazil
[2] State Univ Maringa UEM, Dept Chem, Grp Polymer Mat & Composites, BR-87020900 Maringa, Parana, Brazil
[3] Colorado State Univ CSU, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA
[4] Colorado State Univ CSU, Sch Adv Mat Discovery, Ft Collins, CO 80523 USA
[5] Colorado State Univ CSU, Sch Biomed Engn, Ft Collins, CO 80523 USA
[6] Univ Fed Piaui, Dept Chem, BR-64049550 Teresina, PI, Brazil
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2021年 / 183卷
关键词：
Iota-carrageenan; Polysaccharides; Anti-adhesive coatings; IN-VITRO; IONOTROPIC GELATION; CONTROLLED-RELEASE; STEM-CELLS; COMPLEXES; NANOPARTICLES; SURFACE; DERIVATIVES; AGENT; BEADS;
D O I：
10.1016/j.ijbiomac.2021.04.138
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach. We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth. Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0. PEMs (15-layers) are alternately deposited on the GG film. The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements. The TN/CAR coating provides a hydrophobic (WCA = 127 degrees) and rough surface (R-q - 243 +/- 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78 degrees) with the lowest roughness (R-q = 97 +/- 12 nm). Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria. The films are also cytocompatible. Therefore, they have properties that can be further developed as wound dressings and food packaging. (C) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页码：727 / 742
页数：16
相关论文
共 50 条
  • [1] Preparation and characterization of polyelectrolyte complex of N,N,N-trimethyl chitosan/gellan gum: evaluation for controlled release of ketoprofen
    Mithun, U.
    Vishalakshi, B.
    Karthika, J. S.
    IRANIAN POLYMER JOURNAL, 2016, 25 (04) : 339 - 348
  • [2] Preparation and characterization of polyelectrolyte complex of N,N,N-trimethyl chitosan/gellan gum: evaluation for controlled release of ketoprofen
    U. Mithun
    B. Vishalakshi
    J. S. Karthika
    Iranian Polymer Journal, 2016, 25 : 339 - 348
  • [3] Coating Electrospun Chitosan Nanofibers with Polyelectrolyte Multilayers Using the Polysaccharides Heparin and N,N,N-Trimethyl Chitosan
    Almodovar, Jorge
    Kipper, Matt J.
    MACROMOLECULAR BIOSCIENCE, 2011, 11 (01) : 72 - 76
  • [4] Bioconjugation of quantum-dots with chitosan and N,N,N-trimethyl chitosan
    Mansur, Herman S.
    Mansur, Alexandra A. P.
    Curti, Elisabete
    De Almeida, Mauro V.
    CARBOHYDRATE POLYMERS, 2012, 90 (01) : 189 - 196
  • [5] THE N-PERMETHYLATION OF CHITOSAN AND THE PREPARATION OF N-TRIMETHYL CHITOSAN IODIDE
    MUZZARELLI, RAA
    TANFANI, F
    CARBOHYDRATE POLYMERS, 1985, 5 (04) : 297 - 307
  • [6] Synthesis and structural characterization of N,N,N-trimethyl chitosan
    Kang, Yu
    Liu, Zhi
    Long, Yingyun
    Wang, Baolong
    Yang, Xu
    Sha, Di
    Shi, Kai
    Ji, Xiangling
    Li, Bai
    Liu, Yonggang
    JOURNAL OF APPLIED POLYMER SCIENCE, 2021,
  • [7] Entrapment characteristics of hydrosoluble vitamins loaded into chitosan and N,N,N-trimethyl chitosan nanoparticles
    Douglas de Britto
    Marcia R. de Moura
    Fauze A. Aouada
    Flávia G. Pinola
    Lícia M. Lundstedt
    Odilio B. G. Assis
    Luiz H. C. Mattoso
    Macromolecular Research, 2014, 22 : 1261 - 1267
  • [8] Entrapment characteristics of hydrosoluble vitamins loaded into chitosan and N,N,N-trimethyl chitosan nanoparticles
    de Britto, Douglas
    de Moura, Marcia R.
    Aouada, Fauze A.
    Pinola, Flavia G.
    Lundstedt, Licia M.
    Assis, Odilio B. G.
    Mattoso, Luiz H. C.
    MACROMOLECULAR RESEARCH, 2014, 22 (12) : 1261 - 1267
  • [9] Synthesis, characteristic and antibacterial activity of N, N, N-trimethyl chitosan
    Geng, Xiaodong
    Yang, Ronghua
    Zhou, Yan
    Li, Jianrong
    Huang, Jianying
    Journal of Chinese Institute of Food Science and Technology, 2013, 13 (05) : 117 - 123
  • [10] N,N,N-trimethyl chitosan nanoparticles as a vitamin carrier system
    de Britto, Douglas
    de Moura, Marcia R.
    Aouada, Fauze A.
    Mattoso, Luiz H. C.
    Assis, Odilio B. G.
    FOOD HYDROCOLLOIDS, 2012, 27 (02) : 487 - 493
12345