Heat-Stress-Mitigating Effects of a Protein-Hydrolysate-Based Biostimulant Are Linked to Changes in Protease, DHN, and HSP Gene Expression in Maize

被引：18

作者：

Vaseva, Irina I. ^{[1
]}

Simova-Stoilova, Lyudmila ^{[1
]}

Kostadinova, Anelia ^{[1
]}

Yuperlieva-Mateeva, Bistra ^{[1
]}

Karakicheva, Tania ^{[1
]}

Vassileva, Valya ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Inst Plant Physiol & Genet, Dept Mol Biol & Genet, Acad Georgi Bonchev Str,Bldg 21, Sofia 1113, Bulgaria

来源：

AGRONOMY-BASEL | 2022年 / 12卷 / 05期

关键词：

dehydrins; heat stress; HSPs; proteases; protein-hydrolysate-based biostimulants; Zea mays L; TEMPERATURE STRESS; PLANT TOLERANCE; DROUGHT; GROWTH; MECHANISMS; RESISTANCE; DEHYDRINS; PROLINE; COLD;

D O I：

10.3390/agronomy12051127

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The growth-promoting and heat-mitigating effects of a commercially available protein-hydrolysate-based biostimulant, Kaishi, during the early vegetative stage was investigated by applying it as a foliar spray on soil-grown maize plants or in the nutrient solution of hydroponically grown plants. At 10(-3) dilution, the biostimulant inhibited germination and delayed the growth progress, while at 10(-6) -10(-12) dilutions, it promoted shoot and root growth. Heat stress caused biomass reduction, decreased leaf pigment content and the chlorophyll a/chlorophyll b (chl a/b) ratio, caused starch depletion, and increased lipid peroxidation. Kaishi priming resulted in the substantial mitigation of negative stress effects, maintaining growth, stabilizing pigment content and the chl a/b ratio, restoring the leaf starch content, lowering the malondialdehyde (MDA) level, and significantly increasing the free proline content. The expression profiles of a set of genes coding for heat shock proteins (HSPs), dehydrins (DHNs), and proteases were analysed using qRT-PCR after heat stress exposure. The biostimulant-treated plants had higher transcript levels of certain HSPs, DHNs, and protease-coding genes, which remained stable or increased after the applied stress. The results demonstrate that very low concentrations of the biostimulant exerted stress-mitigating effects that could be linked to organ-specific changes in the gene expression of certain stress-inducible proteins.

引用

页数：20