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14-3-3 Proteins Participate in Light Signaling through Association with PHYTOCHROME INTERACTING FACTORs
被引:19
|作者:
Adams, Eri
[1
]
Diaz, Celine
[1
,2
]
Hong, Jong-Pil
[1
]
Shin, Ryoung
[1
]
机构:
[1] RIKEN Ctr Sustainable Resource Sci, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[2] Univ Autonoma Barcelona, Ctr Res Agr Genom CRAG, E-08193 Barcelona, Spain
来源:
关键词:
PHYTOCHROME INTERACTING FACTOR (PIF);
14-3-3;
protein;
light signaling;
photomorphogenesis;
Arabidopsis thaliana;
plant;
protein-protein interaction;
ARABIDOPSIS-THALIANA;
TRANSCRIPTION FACTOR;
FUNCTIONAL-CHARACTERIZATION;
CHLOROPLAST DEVELOPMENT;
INDUCED PHOSPHORYLATION;
NUCLEAR ACCUMULATION;
A PHOTORECEPTOR;
FACTOR PIF3;
DEGRADATION;
TRANSDUCTION;
D O I:
10.3390/ijms151222801
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
14-3-3 proteins are regulatory proteins found in all eukaryotes and are known to selectively interact with phosphorylated proteins to regulate physiological processes. Through an affinity purification screening, many light-related proteins were recovered as 14-3-3 candidate binding partners. Yeast two-hybrid analysis revealed that the 14-3-3 kappa isoform (14-3-3.) could bind to PHYTOCHROME INTERACTING FACTOR3 (PIF3) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). Further analysis by in vitro pull-down assay confirmed the interaction between 14-3-3. and PIF3. Interruption of putative phosphorylation sites on the 14-3-3 binding motifs of PIF3 was not sufficient to inhibit 14-3-3. from binding or to disturb nuclear localization of PIF3. It was also indicated that 14-3-3. could bind to other members of the PIF family, such as PIF1 and PIF6, but not to LONG HYPOCOTYL IN FAR-RED1 (HFR1). 14-3-3 mutants, as well as the PIF3 overexpressor, displayed longer hypocotyls, and a pif3 mutant displayed shorter hypocotyls than the wild-type in red light, suggesting that 14-3-3 proteins are positive regulators of photomorphogenesis and function antagonistically with PIF3. Consequently, our results indicate that 14-3-3 proteins bind to PIFs and initiate photomorphogenesis in response to a light signal.
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页码:22801 / 22814
页数:14
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