Comparison of an immunochromatography test with multiplex reverse transcription-PCR for rapid diagnosis of respiratory syncytial virus infections

被引：29

作者：

Kuroiwa, Y

Nagai, K

Okita, L

Ukae, S

Mori, T

Hotsubo, T

Tsutsumi, H

机构：

[1] Sapporo Med Univ, Sch Med, Dept Pediat, Chuo Ku, Sapporo, Hokkaido 0608543, Japan

[2] Sapporo Social Insurance Gen Hosp, Dept Pediat, Sapporo, Hokkaido, Japan

[3] NTT Corp, E Sapporo Hosp, Dept Pediat, Sapporo, Hokkaido, Japan

[4] Tonan Hosp, Dept Pediat, Sapporo, Hokkaido, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 10期

关键词：

D O I：

10.1128/JCM.42.10.4812-4814.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new commercial rapid 10-min one-step immunochromatography (IC) test, SAS RSV test, was compared to another IC test, Directigen EZ RSV, employing RT-PCR as the "gold standard" for detecting respiratory syncytial virus. Of 102 clinical samples, 79 were positive by RT-PCR, 66 (82.5%) were positive with the SAS RSV test, and 55 (69.6%) were positive with Directigen EZ RSV. The specificity of the new test was 91.3% (21 of 23), similar to that of Directigen EZ RSV (100% [23 of 23]). This test performs well enough to be used for patient care.

引用

页码：4812 / 4814

页数：3

共 50 条

[1] Diagnosis of respiratory syncytial virus infection: Comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness
Falsey, AR
Formica, MA
Walsh, EE
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) : 817 - 820
[2] Discrimination of respiratory syncytial virus subgroups A and B by reverse transcription-PCR
Gottschalk, J
Zbinden, R
Kaempf, L
Heinzer, I
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) : 41 - 43
[3] Comparison of quantitative reverse transcription-PCR to viral culture for assessment of respiratory syncytial virus shedding
Falsey, AR
Formica, MA
Treanor, JJ
Walsh, EE
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) : 4160 - 4165
[4] Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays
Liao, R. S.
Tomalty, L. L.
Majury, A.
Zoutman, D. E.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (03) : 527 - 532
[5] Evaluation of a nested reverse transcription-PCR assay based on the nucleoprotein gene for diagnosis of spontaneous and experimental bovine respiratory syncytial virus infections
Valarcher, JF
Bourhy, H
Gelfi, J
Schelcher, F
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) : 1858 - 1862
[6] Rapid Virological Diagnosis of Central Nervous System Infections by Use of a Multiplex Reverse Transcription-PCR DNA Microarray
Leveque, Nicolas
Van Haecke, Adrien
Renois, Fanny
Boutolleau, David
Talmud, Deborah
Andreoletti, Laurent
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (11) : 3874 - 3879
[7] Diagnosis of viral respiratory tract infections in children using a reverse transcription-PCR panel
Gilbert, LL
Dakhama, A
Bone, BM
Thomas, EE
Hegele, RG
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) : 140 - 143
[8] Preliminary evaluation of a multiplex reverse transcription-PCR assay combined with a new DNA chip hybridization assay for detecting respiratory syncytial virus
Causse, Manuel
Garcia-Mayorgas, Angel D.
Gutierrez, Juan B.
Casal, Manuel
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (11) : 3811 - 3813
[9] Immunochromatography test for rapid diagnosis of adenovirus respiratory tract infections: Comparison with virus isolation in tissue culture
Tsutsumi, H
Ouchi, K
Ohsaki, M
Yamanaka, T
Kuniya, Y
Takeuchi, Y
Nakai, C
Meguro, H
Chiba, S
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) : 2007 - 2009
[10] Diagnosis and epidemiological surveillance of influenza and respiratory syncytial virus infections:: interest of multiplex PCR
Plouzeau, C.
Paccalin, M.
Beby-Defaux, A.
Giraudeau, G.
Godet, C.
Agius, G.
[J]. MEDECINE ET MALADIES INFECTIEUSES, 2007, 37 (11): : 728 - 733

← 1 2 3 4 5 →