Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus

被引：0
|
作者：
Li, Shikai [1 ,2 ]
Wang, Wei [1 ,2 ]
Yang, Mingju [1 ,2 ]
Huang, Fujiang [1 ,2 ]
Wang, Xue [1 ,2 ]
Zeng, Sheng [1 ,2 ]
Xiang, Yan [1 ,2 ]
Zhou, Lu [1 ,2 ]
机构：
[1] Guizhou Acad Agr Sci, Fisheries Res Inst, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
[2] Engn & Technol Ctr Special Fisheries, 2448 Huaxi Ave, Guiyang 550025, Guizhou, Peoples R China
来源：
CONSERVATION GENETICS RESOURCES | 2022年 / 14卷 / 03期
关键词：
Semilabeo obscurus; SNP; Molecular marker; Germplasm conservation;
D O I：
10.1007/s12686-022-01271-6
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Semilabeo obscurus, as an endangered species, is a certain economic fish in China and Vietnam. However, study on its genetic diversity is still lacking. In this study, 168 candidate SNPs were verified by Sanger-sequencing. Of these, 123 polymorphic SNP markers were successfully genotyped. The observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information conten (PIC) ranged from 0.0968-0.9310 (mean 0.3590), 0.0936-0.5085 (mean 0.3550) and 0.0879-0.3750 (mean 0.2807), respectively. Only 3 SNPs (Sob-016, Sob-050 and Sob-115) were significantly deviated from Hardy-Weinberg equilibrium (P < 0.05). Our study may help researchers better understand the genetic traits of S. obscurus,and provide reference for further protecting and reasonably utilizing its genetic resources.
引用
收藏
页码：257 / 261
页数：5
相关论文
共 50 条
  • [1] Development and characterization of 123 SNPs in an endangered fish Semilabeo obscurus
    Shikai Li
    Wei Wang
    Mingju Yang
    Fujiang Huang
    Xue Wang
    Sheng Zeng
    Yan Xiang
    Lu Zhou
    Conservation Genetics Resources, 2022, 14 : 257 - 261
  • [2] Characterization of the complete mitochondrial genome of an endangered fish Semilabeo obscurus (Cyprinidae; Labeoninae; Semilabeo)
    Wang, Wei
    Li, Shikai
    Liu, Ting
    Wang, Xue
    Zeng, Sheng
    Min, Wenwu
    Zhou, Lu
    CONSERVATION GENETICS RESOURCES, 2019, 11 (02) : 147 - 150
  • [3] Characterization of the complete mitochondrial genome of an endangered fish Semilabeo obscurus (Cyprinidae; Labeoninae; Semilabeo)
    Wei Wang
    Shikai Li
    Ting Liu
    Xue Wang
    Sheng Zeng
    Wenwu Min
    Lu Zhou
    Conservation Genetics Resources, 2019, 11 : 147 - 150
  • [4] Development and characterization of 68 SNPs markers in the critically endangered species, Palea steindachneri
    Zhu, Junxian
    Liu, Pan
    Ji, Liqin
    Hong, Xiaoyou
    Liu, Xiaoli
    Chen, Haigang
    Wei, Chengqing
    Zhu, Xinping
    Chen, Chen
    Li, Wei
    CONSERVATION GENETICS RESOURCES, 2024, 16 (03) : 203 - 207
  • [5] The complete mitochondrial genome of the endangered freshwater fish Odontobutis obscurus from Korea
    Kim, Kang-Rae
    Kwak, Yeong-Ho
    Sung, Mu-Sung
    Lee, Gun Young
    Kim, Yong Hwi
    Bang, In-Chul
    MITOCHONDRIAL DNA PART B-RESOURCES, 2022, 7 (03): : 550 - 551
  • [6] Faecal DNA Metabarcoding for Diet Analysis of Endangered Fish Species, Odontobutis obscurus
    Kim, Kanghui
    You, Kyung-A
    Kim, Jeong-Hui
    Park, Sang-Hyeon
    Baek, Seung-Ho
    Jeong, Kwang-Seuk
    Joo, Gea-Jae
    Jo, Hyunbin
    ANIMALS, 2024, 14 (21):
  • [7] Development and characterization of eighteen microsatellites for endangered fish Huso dauricus
    Lu Cuiyun
    Qianqian Yin
    Lei, Cheng
    Ying, Gu
    Chao, Li
    Xiaowen Sun
    CONSERVATION GENETICS RESOURCES, 2014, 6 (02) : 361 - 365
  • [8] Development and characterization of eighteen microsatellites for endangered fish Huso dauricus
    Lu Cuiyun
    Yin Qianqian
    Cheng Lei
    Gu Ying
    Li Chao
    Sun Xiaowen
    Conservation Genetics Resources, 2014, 6 : 361 - 365
  • [9] Development and characterization of twenty microsatellite markers for the endangered fish Luciobarbus capito
    Geng Longwu
    Lu Cuiyun
    Tong Guangxiang
    Li Chao
    Xu Wei
    CONSERVATION GENETICS RESOURCES, 2012, 4 (04) : 865 - 867
  • [10] Development and characterization of twenty microsatellite markers for the endangered fish Luciobarbus capito
    Geng Longwu
    Lu Cuiyun
    Tong Guangxiang
    Li Chao
    Xu Wei
    Conservation Genetics Resources, 2012, 4 : 865 - 867
12345