Aneuploidy analysis in day 7 human blastocysts produced by in vitro fertilization

被引:25
|
作者
Su, Yu [1 ]
Li, Jian-Jun [1 ]
Wang, Cassie [2 ]
Haddad, Ghassan [3 ,4 ]
Wang, Wei-Hua [2 ,4 ]
机构
[1] Changsha Hosp Maternal & Child Hlth Care, Ctr Reprod Med, Changsha, Hunan, Peoples R China
[2] Vivere Houston Fertil Lab, 2500 Fondren Rd,Suite 350, Houston, TX USA
[3] Houston Fertil Inst, Houston, TX USA
[4] Vivere Hlth, Franklin, TN USA
关键词
Aneuploidy; Delayed blastocyst; Implantation; PGS; PREIMPLANTATION GENETIC DIAGNOSIS; ADVANCED MATERNAL AGE; CHROMOSOME-ABNORMALITIES; EMBRYOS; MOSAICISM; PREGNANCY; SELECTION; COUPLES; WOMEN; PGS;
D O I
10.1186/s12958-016-0157-x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Human embryos produced by in vitro fertilization (IVF) are usually cultured to day 6 after insemination, and good quality of embryos should develop to blastocyst stage at days 5 and 6. However, some embryos develop slowly, thus they may form blastocysts on day 7. Most IVF laboratories culture embryos to day 6 and then discard retarded embryos that do not develop to blastocyst stage. It has been reported that transfer of day 7 blastocysts can yield pregnancy although the rates were very low. In the present study, we evaluated the prevalence of aneuploidy in day 7 human blastocysts and also assessed embryo implantation after transfer of normal euploid blastocysts developed on day 7. Methods: Day 7 blastocysts were biopsied and screened for aneuploidy. Embryo implantation was assessed by transferring of euploid blastocysts. Results: A total of 1966 blastocysts from 367 IVF cycles were biopsied and screened for aneuploidy. It was found that 81.5 % of the patients had days 5 and 6 blastocysts and 18.5 % (68) patients had blastocysts developed on day 7, including 15.3 % had days 5-7 blastocysts and 3.3 % had only day 7 blastocysts. A total of 151 day 7 blastocysts, which accounted for 7.7 % of total blastocysts, were analyzed. It was found that 36.7 % of the blastocysts were euploid and 63.3 % had abnormal chromosomes, including aneuploidy and euploid with partial chromosome deletion. The aneuploidy rate was also maternal age dependent and was as high as 91.7 % in patients who were >= 40 years old. During the study period, transfer of day 7 euploid blastocysts in 15 patients resulted in 2 healthy live births. Conclusion(s): Aneuploidy rates in day 7 human blastocysts produced by IVF are very high. However, good euploid blastocysts have potential to implant and transfer of day 7 euploid blastocysts can result in healthy live birth. It is suggested that day 7 blastocyst culture may be necessary in patients who need aneuploidy screening.
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页数:7
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