Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217

被引：32

作者：

Wada, Jun

Suzuki, Ryuichiro

Fushinobu, Shinya

Kitaoka, Motomitsu

Wakagi, Takayoshi

Shoun, Hirofumi

Ashida, Hisashi

Kumagai, Hidehiko

Katayama, Takane ^{[1
]}

Yamamoto, Kenji

机构：

[1] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan

[2] Kyoto Univ, Grad Sch Studies, Sakyo Ku, Kyoto 6068502, Japan

[3] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

[4] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S1744309107036263

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant galacto-N-biose-/ lacto-N-biose I-binding protein ( GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9 -6.4 and 20 -22%( v/ v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 angstrom for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 angstrom for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 angstrom resolution, respectively.

引用

页码：751 / 753

页数：3

共 3 条

[1] Structural and thermodynamic analyses of solute-binding protein from Bifidobacterium longum specific for core 1 disaccharide and lacto-N-biose I
Suzuki, Ryuichiro
Wada, Jun
Katayama, Takane
Fushinobu, Shinya
Wakagi, Takayoshi
Shoun, Hirofumi
Sugimoto, Hayuki
Tanaka, Akiyoshi
Kumagai, Hidehiko
Ashida, Hisashi
Kitaoka, Motomitsu
Yamamoto, Kenji
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (19) : 13165 - 13173
[2] Crystallization and preliminary X-ray analysis of the Streptomyces olivaceoviridis NgcE binding protein of the ABC transporter for N-acetylglucosamine
Saito, A
Fujimoto, Z
Minami, E
Mizuno, H
Miyashita, K
Schrempf, H
Momma, M
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 2358 - 2360
[3] Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima
Ethayathulla, Abdul S.
Bessho, Yoshitaka
Shinkai, Akeo
Padmanabhan, Balasundaram
Singh, Tej P.
Kaur, Punit
Yokoyama, Shigeyuki
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 498 - 500

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