Extraction of genomic DNA from filamentous fungi in biofilms on water-based paint coatings

被引：13

作者：

Saad, DS ^{[1
]}

Kinsey, GC

Kim, S

Gaylarde, CC

机构：

[1] Univ Fed Santa Maria, Dept Struct & Civil Construct, BR-97105900 Santa Maria, RS, Brazil

[2] CABI Biosci, Egham, Surrey, England

[3] Univ Fed Rio Grande do Sul, Dept Biophys, BR-91501970 Porto Alegre, RS, Brazil

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2004年 / 54卷 / 2-3期

关键词：

biofilm; DNA extraction; fungi; paint; PCR;

D O I：

10.1016/j.ibiod.2004.05.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method has been developed which can be used to extract DNA efficiently from spores of the genera Acremonium, Alternaria, Aureobasidium, Cladosporium and Epicoccum on painted surfaces. The method involves spore lysis by incubation of the specimen with the enzyme Lyticase for 60 min, followed by bead beating for 15 min. DNA is then purified from the lysate with the QIAamp DNA Mini kit. The extracted DNA is of high molecular weight and can be used in PCR for amplification using fungal-specific primers. (C) 2004 Published by Elsevier Ltd.

引用

页码：99 / 103

页数：5

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