Synthesis and characterization of hafnium oxide nanoparticles for bio-safety

In recent years, the perspective of applying hafnium oxide (HfO2) has geared up in various field of medical science such as neutron detection, bioimplants, biosensors, radiotherapy etc., The present study is to synthesis HfO2 nanoparticles, and check its cell viability for in vivo applications. Hafnium oxide nanoparticles of different sizes (8.79, 7.16, 6.78 nm) were synthesized by varying the intervals of stirring time (6 h, 8 h, 12 h) by precipitation method. XRD pattern and Raman spectroscopy revealed that this material crystallizes in a monoclinic structure. SEM images and TEM micrographs showed that the HfO2 NPs were spherical in shape with an average particle size of below 10 nm. The EDAX spectrum showed that the synthesized nanoparticles were HfO2. 3T3 fibroblast cell lines were chosen for cytotoxic study as it mimics the human cells. The aim of this study is to compare the toxicity of different sizes of HfO2 nanoparticles on interaction with 3T3 fibroblast cell lines. From this study we could infer that smaller sized nanoparticles (6.78 and 7.17 nm) have 86% cell viability even at the concentration of 2500 mu g/mL.