Ursolic acid from Prunella vulgaris L. efficiently inhibits IHNV infection in vitro and in vivo

被引：43

作者：

Li, Bo-Yang ^{[1
]}

Hu, Yang ^{[2
]}

Li, Jian ^{[2
]}

Shi, Kai ^{[2
]}

Shen, Yu-Feng ^{[2
]}

Zhu, Bin ^{[2
]}

Wang, Gao-Xue ^{[2
]}

机构：

[1] Northwest A&F Univ, Coll Chem & Pharm, Xinong Rd 22nd, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Coll Anim Sci & Technol, Xinong Rd 22nd, Yangling 712100, Shaanxi, Peoples R China

来源：

VIRUS RESEARCH | 2019年 / 273卷

基金：

中国国家自然科学基金;

关键词：

Ursolic acid; Antiviral activity; Rainbow trout; IHNV; HEMATOPOIETIC NECROSIS VIRUS; RAINBOW-TROUT; ANTIVIRAL ACTIVITY; IMMUNE-RESPONSES; OLEANOLIC ACID; E-SELECTIN; EXPRESSION; AUTOPHAGY; EXTRACT; REPLICATION;

D O I：

10.1016/j.virusres.2019.197741

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Infectious hematopoietic necrosis virus (IHNV) is a fish viral pathogen that causes severe disease and huge economic losses in the salmonid aquaculture industry. However, anti-IHNV drugs currently are scarce. For the purpose of seeking out anti-IHNV drugs, the anti-IHNV activities of 32 medicinal plants were investigated by using epithelioma papulosum cyprini (EPC) cells. Among these plants, Prunella vulgaris L. (PVL) showed the strongest inhibition on IHNV replication with an inhibitory percentage of 99.3% at the concentration 100 mg/L. Further studies demonstrated that ursolic acid (UA), a major constituent of PVL, also showed a highly effective anti-IHNV activity. The half-maximal inhibitory concentration (IC50) at 72 h of UA on IHNV was 8.0 mu M. Besides, UA could significantly decrease cytopathic effect (CPE) and the viral titer induced by IHNV in EPC cells. More importantly, UA also showed a strong anti-IHNV activity in vivo, as indicated by increasing the survival rate of rainbow trout and inhibiting viral gene expression. Intraperitoneal injection of UA increased the relative percentage of survival of rainbow trout by 18.9% and inhibited IHNV glycoprotein mRNA expression by > 90.0% in the spleen at the 1st-day post-infection. Altogether, UA was expected to be a therapeutic agent against IHNV infection in aquaculture.

引用

页数：8