Urinary excretion of 5-S-cysteinyldopa and 6-hydroxy-5-methoxyindole-2-carboxylic acid in children

5-S-Cysteinyldopa (5SCD) and 6-hydroxy-5-methoxyindole-2-carboxylic acid (6H5MI2C) are formed during biosynthesis of melanins. They are used as indicators of pigment formation and markers of melanoma progression in adults and could possibly be used as markers of activity, growth and even malignant transformation in large pigmented naevi in children. We investigated the urinary excretion of these pigment precursor metabolites from 136 children, 5 to 15 Sears of age. The mean 5SCD excretion mas 38.1 mu mol/mol creatinine. A significant age-related decrease from a mean of 60.4 mu mol/mol creatinine at 5 years of age to 28.0 mu mol/mol creatinine at age 15 was found. In a reference group (29 adults, 20-33 years of age) the mean excretion mas 48.9 mu mol/mol creatinine. The mean excretion of 6H5MI2C was 42.8 mu mol/mol creatinine at 5 Sears of age and 26.1 mu mol/mol creatinine at the age of 15. The mean value for the young adults was 33.4 mu mol/mol creatinine. No correlation between the mean excretion of 5SCD and 6H5MI2C was demonstrated. We suggest an upper reference level of 90 mu mol/mol creatinine for the excretion of 5SCD in the age group 5-11 years and of 60 mu mol/mol creatinine in the age group 13-15 years. Corresponding figures for the indole 6H5MI2C are 70 and 60 mu mol/mol creatinine. The establishment of reference values in children mill make it possible to use SSCD and 6H5MI2C measurements as diagnostic tools, indicating growth or malignant transformation in giant melanocytic naevi during childhood.