A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

被引：9
|
作者：
Xia, Hongyan [1 ,2 ]
Liu, Lihong [1 ]
Nordengrahn, Ann [3 ]
Kiss, Istvan [1 ]
Merza, Malik [3 ]
Eriksson, Ronnie [4 ]
Blomberg, Jonas [4 ]
Belak, Sandor [1 ,2 ]
机构：
[1] Natl Vet Inst, Joint R&D Div Virol, SE-75189 Uppsala, Sweden
[2] Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SE-75189 Uppsala, Sweden
[3] Svanova Biotech AB, SE-75183 Uppsala, Sweden
[4] Dept Med Sci, Lab Sect Virol, SE-75185 Uppsala, Sweden
来源：
JOURNAL OF VIROLOGICAL METHODS | 2010年 / 168卷 / 1-2期
关键词：
Luminex; Microsphere immunoassay; BVDV; BVDV; INFECTION; ASSAY; ERADICATION; EPITOPES; PROTEIN; PLASMA; STRAIN;
D O I：
10.1016/j.jviromet.2010.04.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：18 / 21
页数：4
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