Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

被引：102

作者：

Dockter, Christoph ^{[1
]}

Gruszka, Damian ^{[2
]}

Braumann, Ilka ^{[1
]}

Druka, Arnis ^{[3
]}

Druka, Ilze ^{[3
]}

Franckowiak, Jerome ^{[4
]}

Gough, Simon P. ^{[1
]}

Janeczko, Anna ^{[5
]}

Kurowska, Marzena ^{[2
]}

Lundqvist, Joakim ^{[1
]}

Lundqvist, Udda ^{[6
]}

Marzec, Marek ^{[2
]}

Matyszczak, Izabela ^{[1
]}

Muller, Andre H. ^{[1
]}

Oklestkova, Jana ^{[7
,8
]}

Schulz, Burkhard ^{[9
]}

Zakhrabekova, Shakhira ^{[1
]}

Hansson, Mats ^{[1
]}

机构：

[1] Carlsberg Lab, DK-1799 Copenhagen V, Denmark

[2] Univ Silesia, Fac Biol & Environm Protect, Dept Genet, PL-40032 Katowice, Poland

[3] James Hutton Inst, Dundee DD2 5DA, Scotland

[4] Hermitage Res Facil, Dept Agr Fishery & Forestry Agrisci Queensland, Warwick, Qld 4370, Australia

[5] Polish Acad Sci, Inst Plant Physiol, PL-30239 Krakow, Poland

[6] Nord Genet Resource Ctr, SE-23053 Alnarp, Sweden

[7] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Lab Growth Regulators, CZ-78371 Olomouc, Czech Republic

[8] Acad Sci Czech Republ, Inst Expt Bot, CZ-78371 Olomouc, Czech Republic

[9] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA

来源：

PLANT PHYSIOLOGY | 2014年 / 166卷 / 04期

基金：

美国国家科学基金会;

关键词：

GIBBERELLIN; RECEPTOR; RICE; MUTANT; WHEAT; ARABIDOPSIS; MUTATIONS; GENOME; PERCEPTION; MECHANISM;

D O I：

10.1104/pp.114.250738

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID-INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroid-related genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars.

引用

页码：1912 / 1927

页数：16