Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue

被引：11
|
作者：
Lima, L. F. [1 ]
Rocha, R. M. P. [1 ]
Alves, A. M. C. V. [1 ]
Carvalho, A. A. [1 ]
Chaves, R. N. [2 ]
Lopes, C. A. P. [1 ]
Bao, S. N. [3 ]
Campello, C. C. [1 ]
Rodrigues, A. P. R. [1 ]
Figueiredo, J. R. [1 ]
机构：
[1] Univ Estadual Ceara, Fac Vet Med, Lab Manipulat Oocytes & Preantral Follicles LAMOF, Fortaleza, Ceara, Brazil
[2] Univ Fortaleza, Hlth Sci Ctr, Lab Morphofunct, Fortaleza, Ceara, Brazil
[3] Univ Brasilia, Dept Cell Biol, Lab Electron Microscopy, Brasilia, DF, Brazil
来源：
COMPLEMENTARY THERAPIES IN MEDICINE | 2016年 / 25卷
关键词：
Homeopathy; Ovine; Ovary; Reproduction; LABORATORY RESEARCH; GROWTH-FACTOR; HOMEOPATHY; EXPRESSION; RECEPTORS; SURVIVAL;
D O I：
10.1016/j.ctim.2015.12.016
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Objective: This study compared 2 types of recombinant follicle stimulating hormone (rFSH): diluted and diluted/dynamized, on in vitro development of ovine follicles. Methods: In experiment 1, ovarian fragments were cultured for 1 or 7 days in alpha-MEM+ in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH-ultradiluted and succussioned), alone or in combination, was studied. Results: In experiment 1, compared to control, 50 ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture (P < 0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture (P < 0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture (P < 0.05). Conclusion: In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：39 / 44
页数：6
相关论文
共 7 条
  • [1] Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro
    de Lima, Laritza Ferreira
    Pedrosa Rocha, Rebeca Magalhaes
    Costa Vasconcelos Alves, Anelise Maria
    Alves Saraiva, Marcia Viviane
    Araujo, Valdevane Rocha
    Teixeira Lima, Isadora Machado
    Pinho Lopes, Claudio Afonso
    Bao, Sonia Nair
    Campello, Claudio Cabral
    Ribeiro Rodrigues, Ana Paula
    de Figueiredo, Jose Ricardo
    [J]. HOMEOPATHY, 2013, 102 (01) : 41 - 48
  • [2] Ultra-diluted/dynamized doxorubicin reduces the toxicity caused by doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue
    de Andrade, Ramyres Diego Lima
    Palomino, Gaby Judith Quispe
    de Queiroz, Isabor Sales Marinho
    da Silva, Ana Flavia Bezerra
    Ferreira, Anna Clara Accioly
    Alves, Benner Geraldo
    de Morais, Selene Maia
    Rodrigues, Ana Paula Ribeiro
    de Lima, Laritza Ferreira
    de Figueiredo, Jose Ricardo
    [J]. REPRODUCTIVE TOXICOLOGY, 2024, 129
  • [3] Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles
    Calongos, Giannina
    Hasegawa, Akiko
    Komori, Shinji
    Koyama, Koji
    [J]. FERTILITY AND STERILITY, 2008, 89 : 1482 - 1489
  • [4] Interactions between different media and follicle-stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacu Linneaus, 1758)
    Lima, G. L.
    Luz, V. B.
    Lima, L. F.
    Rocha, R. M. P.
    Castro, S. V.
    Castelo, T. S.
    Rodrigues, A. P. R.
    Figueiredo, J. R.
    Silva, A. R.
    [J]. REPRODUCTION IN DOMESTIC ANIMALS, 2018, 53 (04) : 880 - 888
  • [5] Expression of follicle-stimulating hormone receptor (FSHR) in goat ovarian follicles and the impact of sequential culture medium on in vitro development of caprine preantral follicles
    Saraiva, M. V. A.
    Celestino, J. J. H.
    Araujo, V. R.
    Chaves, R. N.
    Almeida, A. P.
    Lima-Verde, I. B.
    Duarte, A. B. G.
    Silva, G. M.
    Martins, F. S.
    Bruno, J. B.
    Matos, M. H. T.
    Campello, C. C.
    Silva, J. R. V.
    Figueiredo, J. R.
    [J]. ZYGOTE, 2011, 19 (03) : 205 - 214
  • [6] Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles
    Rossetto, R.
    Lima-Verde, I. B.
    Matos, M. H. T.
    Saraiva, M. V. A.
    Martins, F. S.
    Faustino, L. R.
    Araujo, V. R.
    Silva, C. M. G.
    Name, K. P. O.
    Bao, S. N.
    Campello, C. C.
    Figueiredo, J. R.
    Blume, H.
    [J]. DOMESTIC ANIMAL ENDOCRINOLOGY, 2009, 37 (02) : 112 - 123
  • [7] Basal and induced progesterone and oestradiol secretion from human granulosa-luteal cells obtained during in-vitro fertilization cycles: a comparison between the effects of ovulation induction using recombinant follicle stimulating hormone (rFSH) and pure urinary FSH
    Dirnfeld, M
    Fruchter, O
    Koifman, M
    Calderon, I
    Yshai, D
    Abramovici, H
    [J]. HUMAN REPRODUCTION, 1999, 14 : 297 - 297
1