Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India

. - Prosopis cineraria Druce (khejri), a member of the Fabaceae family, is an essential legu-minous plant and agri-horticultural crop, which helps to protect the ecology of desert and semi-arid areas. Using the public domain RNA-seq dataset, we aimed to reveal the viral spectrum of khejri, which is still unexplored. Following quality control, 111,497 contigs (> 200 nt) were created by de novo assembly of 24,081,663 reads. The presence of one contig of 10316 nt related to Papaya ringspot virus (PRSV) with sequence identity 83.20%-93.21% was identified through BLASTx analysis, and 3,650 (0.02%) reads were mapped using full-length PRSV sequence as reference. Pairwise sequencing analysis revealed that the nucleotide and amino acid sequences of the complete genome sequence (BK061298) shared 84.30%-95.54% and 86.38%-96.48% similarity with other PRSV genomes, respectively. Phylogenetic analysis revealed that this virus isolate is closely related to PRSV isolate WB (host: Carica papaya; Acc. No. LC482263). Our findings show that Prosopis cineraria (khejri) is a novel host plant of PRSV in India by utilizing khejri transcriptome data and bioinformatics analysis.