Bifidobacterium aquikefiri sp nov., isolated from water kefir

被引:41
|
作者
Laureys, David [1 ,2 ]
Cnockaert, Margo [1 ]
De Vuyst, Luc [2 ]
Vandamme, Peter [1 ]
机构
[1] Univ Ghent, Fac Sci, Dept Biochem & Microbiol, Lab Microbiol, KL Ledeganckstr 35, B-9000 Ghent, Belgium
[2] Vrije Univ Brussel, Fac Sci & Bioengn Sci, Res Grp Ind Microbiol & Food Biotechnol, Pleinlaan 2, B-1050 Brussels, Belgium
关键词
DNA; LACTOBACILLUS; DATABASE; MILK;
D O I
10.1099/ijsem.0.000877
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel Bifidobacterium, strain LMG 28769(T), was isolated from a household water kefir fermentation process. Cells were Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, oxidase-negative and facultatively anaerobic short rods. Analysis of its 16S rRNA gene sequence revealed Bifidobacterium crudilactis and Bifidobacterium psychraerophilum (97.4 and 97.1 % similarity towards the respective type strain sequences) as nearest phylogenetic neighbours. Its assignment to the genus Bifidobacterium was confirmed by the presence of fructose 6-phosphate phosphoketolase activity. Analysis of the hsp60 gene sequence revealed very low similarity with nucleotide sequences in the NCBI nucleotide database. The genotypic and phenotypic analyses allowed the differentiation of strain LMG 28769(T) from all recognized Bifidobacterium species. Strain LMG 28769(T) (=CCUG 67145(T) =R 54638(T)) therefore represents a novel species, for which the name Bifidobacterium aquikefiri sp. nov. is proposed.
引用
收藏
页码:1281 / 1286
页数:6
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